Bacterial cytoplasmic membrane permeability assay using ion-selective electrodes

Journal of Microbiological Methods
Chie OhmizoTakashi Katsu

Abstract

We used K(+) and tetraphenylphosphonium (TPP(+)) electrodes simultaneously to evaluate the ability of antimicrobial peptides to form channels (or more generally to increase permeability) and to abolish membrane potential in bacterial cytoplasmic membranes in situ. Such evaluations are usually made independently by colorimetric monitoring of the hydrolysis of a chromogenic substrate by a cytoplasmic enzyme or by fluorimetric determination of membrane depolarization using a membrane potential-sensitive dye. In the present study, the K(+) electrode was used to evaluate channel-forming ability by monitoring the efflux of K(+) originally present in the cytoplasm of bacteria, while the TPP(+) electrode was used to examine membrane depolarization causing the efflux of TPP(+) accumulated in the cytoplasm of bacteria dependent on membrane potential. Thus, the combination of these two electrodes enabled us to clarify how the peptide-induced formation of ion channels is involved in disrupting the energy-generating system in situ.

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Citations

Jun 9, 2010·Folia Microbiologica·Z MolatováM Marounek
Jan 4, 2012·Photochemistry and Photobiology·Hisato KatoTakashi Katsu
Oct 19, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hisato KatoTakashi Katsu
Mar 8, 2016·Journal of Ethnopharmacology·Karuppusamy ArunachalamDomingos Tabajara de Oliveira Martins
Sep 8, 2009·International Journal of Food Microbiology·F M CamposT A Hogg
Jun 24, 2015·Journal of Ethnopharmacology·Darley Maria OliveiraDomingos Tabajara de Oliveira Martins
Apr 20, 2016·Journal of Microbiological Methods·Sang-Jin SuhLaura Silo-Suh
Apr 8, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Keiko KomagoeTakashi Katsu
Jan 4, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Javad Sharifi-RadMarcello Iriti
Jun 13, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hisato KatoTakashi Katsu
Jun 1, 2010·Probiotics and Antimicrobial Proteins·Christopher MurdockKarl R Matthews
Mar 1, 2012·Probiotics and Antimicrobial Proteins·Yen Nee TanMohd Khan Ayob

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