Bacterial diversities on unaged and aging flue-cured tobacco leaves estimated by 16S rRNA sequence analysis

Applied Microbiology and Biotechnology
Jingwen HuangKe-Qin Zhang

Abstract

Flue-cured tobacco leaves (FCTL) contain abundant bacteria, and these bacteria play very important roles in the tobacco aging process. However, bacterial communities on aging FCTL are not fully understood. In this study, the total microbial genome DNA of unaged and aging flue-cured tobacco K326 were isolated using a culture-independent method, and the bacterial communities were investigated by restriction fragment length polymorphism analysis. Comparison of the number of operational taxonomic units (OTUs) between the cloned libraries from the unaged and aging FCTL showed that the microbial communities between the two groups were different. Fifty and 42 OTUs were obtained from 300 positive clones in unaged and aging FCTL, respectively. Twenty-seven species of bacteria exist in both the unaged and aging FCTL, Bacillus spp. and Pseudomonas spp. were two dominant genera in FCTL. However, 23 bacterial species were only identified from the unaged FCTL, while 15 species were only identified from the aging FCTL. Interestingly, more uncultured bacteria species were found in aging FCTL than in unaged FCTL.

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Citations

Jun 11, 2011·Applied Microbiology and Biotechnology·Can SuJinkui Yang
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Lei ZhaoWeihong Zhong
Jul 21, 2011·Journal of Oncology·John L Pauly, Geraldine Paszkiewicz
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Mar 15, 2012·Bulletin of Environmental Contamination and Toxicology·Xiuguo WangFenglong Wang
Jun 20, 2013·Bulletin of Environmental Contamination and Toxicology·Xiuguo WangCanping Pan
Mar 26, 2015·Applied Microbiology and Biotechnology·Jianli LiuJinkui Yang
Aug 22, 2014·Applied Microbiology and Biotechnology·Huagui LiuWeihong Zhong
Jan 2, 2020·MicrobiologyOpen·Jiaxi ZhouXiao Zou
Feb 12, 2017·Applied Microbiology and Biotechnology·Jianbin YeXuepeng Yang
Oct 5, 2017·World Journal of Microbiology & Biotechnology·Man ZhouXin Lü
Apr 23, 2017·Applied Microbiology and Biotechnology·Eoghan M SmythAmy R Sapkota
Sep 6, 2020·The Science of the Total Environment·Xiaoyulong ChenTomislav Cernava
Jun 11, 2021·Applied Microbiology and Biotechnology·A J Rivera, R E Tyx
Aug 1, 2021·Applied Microbiology and Biotechnology·Akanksha Vishwakarma, Digvijay Verma
Nov 19, 2021·Applied Microbiology and Biotechnology·Jianbin YeZhan Zhang

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