Bacterial diversity associated with wild caught Anopheles mosquitoes from Dak Nong Province, Vietnam using culture and DNA fingerprint

PloS One
Chung Thuy NgoS Manguin

Abstract

Microbiota of Anopheles midgut can modulate vector immunity and block Plasmodium development. Investigation on the bacterial biodiversity in Anopheles, and specifically on the identification of bacteria that might be used in malaria transmission blocking approaches, has been mainly conducted on malaria vectors of Africa. Vietnam is an endemic country for both malaria and Bancroftian filariasis whose parasitic agents can be transmitted by the same Anopheles species. No information on the microbiota of Anopheles mosquitoes in Vietnam was available previous to this study. The culture dependent approach, using different mediums, and culture independent (16S rRNA PCR - TTGE) method were used to investigate the bacterial biodiversity in the abdomen of 5 Anopheles species collected from Dak Nong Province, central-south Vietnam. Molecular methods, sequencing and phylogenetic analysis were used to characterize the microbiota. The microbiota in wild-caught Anopheles was diverse with the presence of 47 bacterial OTUs belonging to 30 genera, including bacterial genera impacting Plasmodium development. The bacteria were affiliated with 4 phyla, Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria, the latter being the dominant phylu...Continue Reading

References

Jan 1, 1992·Memórias do Instituto Oswaldo Cruz·M CoosemansP Carnevale
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E F DeLong
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Feb 1, 1996·The American Journal of Tropical Medicine and Hygiene·C B PumpuniJ C Beier
Sep 1, 1996·Nucleic Acids Research·Y Van de PeerR De Wachter
Jun 6, 1998·Journal of Medical Entomology·S C StraifJ C Beier
May 24, 2001·Molecular Biology and Evolution·D Posada, K A Crandall
Jun 28, 2001·Journal of Clinical Microbiology·M DrancourtD Raoult
Jul 31, 2002·Applied and Environmental Microbiology·Jean-Claude OgierAgnes Delacroix-Buchet
Aug 29, 2003·Journal of Medical Entomology·Lilia Gonzalez-CeronJuan E Hernandez-Avila
Dec 1, 2004·Annual Review of Genetics·Christian S RiesenfeldJo Handelsman
Sep 15, 2005·Systematic and Applied Microbiology·J F BernardetJ Swings
Oct 18, 2005·Trends in Parasitology·Patricia AzambujaNorman A Ratcliffe
Feb 2, 2006·International Journal of Systematic and Evolutionary Microbiology·Peter KämpferIngrid Faye
Jun 20, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·C WaltonR K Butlin
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Guido FaviaDaniele Daffonchio
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Marco VenturaDouwe van Sinderen
Jan 8, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S ManguinM Coosemans
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
May 31, 2008·Advances in Experimental Medicine and Biology·Guido FaviaDaniele Daffonchio
Nov 1, 2008·The Journal of Microbiology·Seong Woon RohJin-Woo Bae
Dec 17, 2008·Current Biology : CB·Claudia DamianiGuido Favia
Jul 25, 2009·International Journal of Systematic and Evolutionary Microbiology·Vijay A K B GundiBernard La Scola
Aug 19, 2009·Journal of Microbiological Methods·Laurent RoudièreEstelle Jumas-Bilak
Nov 28, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S ManguinT Chareonviriyaphap
Jan 12, 2010·International Journal of Systematic and Evolutionary Microbiology·Wei ZhangMing Chen
Mar 27, 2010·The Journal of General and Applied Microbiology·Jintana KommaneeYuzo Yamada
Jun 1, 2010·International Journal of Systematic and Evolutionary Microbiology·Gunnar SturmJohannes Gescher
Jun 24, 2010·Microbial Ecology·Claudia DamianiGuido Favia
May 14, 2011·Science·Chris M CirimotichGeorge Dimopoulos
Nov 22, 2011·Acta Tropica·Rashad Abdul-GhaniMogeeb S Alabsi
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sibao WangMarcelo Jacobs-Lorena

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Citations

Jan 15, 2016·Parasites & Vectors·Ephantus J MuturiMadhura H Siddappaji
Jan 10, 2017·Frontiers in Microbiology·Chung T NgoEstelle Jumas-Bilak
Mar 21, 2018·Microbiome·Morgane GuéganClaire Valiente Moro
Oct 26, 2018·Malaria Journal·Devaiah Monnanda KalappaNeena Valecha
May 24, 2017·Parasitology Research·Sina ZotzmannSven Klimpel
Sep 21, 2018·Frontiers in Cellular and Infection Microbiology·Ivet A YordanovaSusanne Hartmann
Jun 9, 2020·Frontiers in Microbiology·Krajana TainchumSylvie Manguin
May 24, 2021·Malaria Journal·Bianca E SilvaYael L Dahan-Moss

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
Modeltest
Analytic Rarefaction
TTGE
Seqmatch

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