Bacterial diversity in synovial fluids of patients with TMD determined by cloning and sequencing analysis of the 16S ribosomal RNA gene

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Wei SunNatsuki Segami

Abstract

The purpose of this study was to examine the presence of bacteria in synovial fluids from patients with temporomandibular joint disorder (TMD) by polymerase chain reaction (PCR) targeting the 16S ribosomal RNA (rRNA) gene, followed by cloning and sequencing. Universal bacterial primers were used to amplify 16S rRNA genes in 28 synovial fluid samples from 27 patients with TMD (TMD group) as well as control subjects: 5 patients with dislocation of TMJ (non-TMD group). Subsequently, PCR amplicons were purified and cloned. Partial 16S rRNA sequences of the cloned insert were used to determine the species identities or closest relative by comparison with known sequences using GeneBank. Nineteen (67.86%) of 28 samples from patients in the TMD group were identified with the presence of bacterial DNA by PCR. The 5 control samples from the nonTMD group were all negative in this study. After cloning and sequencing, a total of 11 bacterial species was detected, including Granulicatella adiacen (6 samples), Pseudomonas sp. (6 samples), Methylobacterium sp. (5 samples), and Beta proteobacterium (4 samples). Other species, such as Acidovorax sp., Bradyrhizobium sp., Sphingomonas, Streptococcus, Leptothrix sp. oral clone, Thiobacillus denitri...Continue Reading

References

Jun 15, 1999·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·C H HenryL M Wolford
Sep 12, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·N SegamiK I Murakami
May 31, 2002·Journal of Clinical Microbiology·B J PasterF E Dewhirst
Oct 31, 2003·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Sang-Jung KimSeong-Gon Kim
Apr 16, 2004·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Diana I PaegleLena Grillner
Nov 24, 2006·Journal of Clinical Microbiology·Sheena M JohnsonDavid Collins

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Citations

Jan 17, 2012·International Journal of Oral and Maxillofacial Surgery·M McIntosh, G Dimitroulis
Sep 26, 2014·Cranio : the Journal of Craniomandibular Practice·Jorge A LearretaEsteban E Barrientos
Aug 5, 2017·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Maureen BoostZhaoran Wang

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