Bacterial DNA and immunostimulatory CpG oligonucleotides trigger maturation and activation of murine dendritic cells

European Journal of Immunology
T SparwasserH Wagner

Abstract

Bacterial DNA and immunostimulatory (i.s.) synthetic CpG-oligodeoxynucleotides (ODN) act as adjuvants for Th1 responses and cytotoxic T cell responses to proteinaceous antigens. Dendritic cells (DC) can be referred to as "nature's adjuvant" since they display the unique capacity to sensitize naive T cells. Here, we demonstrate that bacterial DNA or i.s. CpG-ODN cause simultaneous maturation of immature DC and activation of mature DC to produce cytokines. These events are associated with the acquisition of professional antigen-presenting cell (APC) function. Unfractionated murine bone marrow-derived DC and FACS-fractionated MHC class IIlow (termed immature DC) or MHC class IIhigh populations (termed mature DC) were stimulated with bacterial DNA or i.s. CpG-ODN. Similar to lipopolysaccharide, i.s. CpG-ODN caused up-regulation of MHC class II, CD40 and CD86, but not CD80 on immature and mature DC. In parallel both DC subsets were activated to produce large amounts of IL-12, IL-6 and TNF-alpha. CpG-ODN-activated DC displayed professional APC function in allogeneic mixed lymphocyte reaction and in staphylococcal enterotoxin B-driven naive T cell responses. We interpret these findings to mean that bacterial DNA and i.s. CpG-ODN cause...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·R M Steinman
Jan 1, 1989·Cold Spring Harbor Symposia on Quantitative Biology·C A Janeway
Oct 1, 1994·The Journal of Experimental Medicine·C CauxJ Banchereau
Apr 6, 1995·Nature·A M KriegD M Klinman
Aug 1, 1993·The Journal of Experimental Medicine·N BhardwajR M Steinman
Jan 1, 1996·The Journal of Experimental Medicine·X MaG Trinchieri
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·D M KlinmanA M Krieg
Apr 1, 1996·The Journal of Experimental Medicine·G Süss, K Shortman
Oct 1, 1996·The Journal of Experimental Medicine·T De SmedtM Moser
Apr 1, 1997·International Archives of Allergy and Immunology·G SchulerN Romani
Mar 27, 1997·Nature·T SparwasserH Wagner
Apr 1, 1997·Immunological Reviews·R M SteinmanK Inaba
Apr 1, 1997·Immunological Reviews·J SprentS Sun
Dec 31, 1997·The Journal of Experimental Medicine·R S ChuC V Harding
Dec 5, 1997·Immunology Today·W O Weigle, C G Romball
Feb 17, 1998·European Journal of Immunology·G B LipfordH Wagner

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Citations

Dec 13, 2006·Clinical & Developmental Immunology·Klara K ErikssonVolker Thiel
Nov 9, 2000·European Journal of Immunology·D SchattenbergJ Seissler
Dec 18, 2001·European Journal of Immunology·S RothenfusserG Hartmann
Sep 18, 2010·Inflammatory Bowel Diseases·Claudia HofmannFlorian Obermeier
May 15, 2007·Immunologic Research·Arpita S Bharadwaj, Devendra K Agrawal
Aug 30, 2012·Archivum Immunologiae Et Therapiae Experimentalis·Swaminathan SethuJean Sathish
Feb 19, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Claudia Danzer, Jochen Mattner
Aug 17, 2000·Springer Seminars in Immunopathology·A A HornerE Raz
Aug 17, 2000·Springer Seminars in Immunopathology·M J McCluskieH L Davis
Aug 17, 2000·Springer Seminars in Immunopathology·A M Krieg, A K Yi
Aug 17, 2000·Springer Seminars in Immunopathology·H Liang, P E Lipsky
Aug 17, 2000·Springer Seminars in Immunopathology·D S Pisetsky
Aug 17, 2000·Springer Seminars in Immunopathology·G J Weiner
Aug 17, 2000·Springer Seminars in Immunopathology·H WagnerH Häcker
Aug 17, 2000·Springer Seminars in Immunopathology·H WagnerG B Lipford
Aug 17, 2000·Springer Seminars in Immunopathology·H KobayashiE Raz
Aug 17, 2000·Springer Seminars in Immunopathology·J C Vogel, M C Udey
Aug 17, 2000·Springer Seminars in Immunopathology·S SunJ Sprent
Aug 17, 2000·Springer Seminars in Immunopathology·J Van Uden, E Raz
Oct 28, 2008·Clinical and Experimental Medicine·Jiong WangRong-Yu Liu
Mar 30, 2010·Fish Physiology and Biochemistry·Christopher Marlowe A CaipangViswanath Kiron
Nov 20, 2010·Pharmaceutical Research·Filipa LebreOlga Borges
Mar 17, 2004·Current Opinion in Immunology·Scott AntoniaJeffrey S Weber
May 25, 2005·Veterinary Immunology and Immunopathology·Masashi HashimotoAtsuhiko Hasegawa
Jun 5, 2003·International Journal for Parasitology·Manmohan Singh, Derek T O'Hagan
Nov 11, 1999·Journal of Immunological Methods·U A HasanW J Morrow
Jun 8, 2001·Mechanisms of Ageing and Development·C Gravekamp
Jun 24, 2003·Developmental and Comparative Immunology·Hang XieRobert A Heckert
Dec 14, 2001·Molecular Immunology·O B EnglerA Cerny
Apr 12, 2002·Veterinary Immunology and Immunopathology·Y Van der StedeB M Goddeeris
Jan 1, 2003·Veterinary Immunology and Immunopathology·Dirk Werling, Thomas W Jungi
Dec 30, 1999·Veterinary Immunology and Immunopathology·W C BrownD M Estes

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