Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice.

PloS One
Keith A MartinezJörn-Hendrik Weitkamp

Abstract

Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it's taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined the presence of bacterial DNA in the intestines and placentas of fetal mice at gestational day 17 (n = 13). These were compared to newborn intestines (n = 15), maternal sites (mouth, n = 6; vagina, n = 6; colon, n = 7; feces, n = 8), and negative controls to rule out contamination. The V4 region of the bacterial 16S rRNA gene indicated a pattern of bacterial DNA in fetal intestine similar to placenta but with higher phylogenetic diversity than placenta or newborn intestine. Firmicutes were the most frequently assignable phylum. SourceTracker analysis suggested the placenta as the most commonly identifiable origin for fetal bacterial DNA, but also over 75% of fetal gut genera overlapped with maternal oral and vaginal taxa but not with maternal or newborn feces. These data provide evidence for the presence of bacterial DNA in the mouse fetus.

References

Sep 28, 2005·Current Microbiology·Esther JiménezJuan M Rodríguez
Aug 3, 2006·Pediatrics·John PendersEllen E Stobberingh
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Feb 19, 2008·Research in Microbiology·Esther JiménezJuan M Rodríguez
Oct 31, 2008·Journal of Clinical Microbiology·Yiping W HanCatalin S Buhimschi
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Maria G Dominguez-BelloRob Knight
Sep 10, 2010·The ISME Journal·Catherine LozuponeRob Knight
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Martin A KriegelDiane Mathis
Mar 29, 2013·The ISME Journal·Ida Gisela Pantoja-FelicianoMaria Gloria Dominguez-Bello
Aug 27, 2013·PLoS Biology·Lisa J Funkhouser, Seth R Bordenstein
Mar 13, 2014·PloS One·Alexandria N ArdissoneJosef Neu
Mar 19, 2014·Gut Microbes·Joann Romano-KeelerJörn-Hendrik Weitkamp
Jun 21, 2014·AMB Express·Helena JunickeRobbert Kleerebezem
Aug 12, 2014·Cancer Prevention Research·Joseph P ZackularPatrick D Schloss
Nov 15, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Diana A ChernikovaJuliette C Madan
Dec 11, 2014·The Journal of Obstetrics and Gynaecology Research·Ranmalee AmarasekaraVajira H W Dissanayake
Jan 6, 2015·American Journal of Obstetrics and Gynecology·Kathleen M AntonyKjersti Aagaard
Mar 12, 2016·American Journal of Obstetrics and Gynecology·Amanda L PrinceKjersti M Aagaard
Aug 10, 2016·Genome Medicine·Derrick M ChuKjersti M Aagaard

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Citations

Oct 7, 2019·BJOG : an International Journal of Obstetrics and Gynaecology·J L O'CallaghanE S Pelzer
Sep 4, 2019·JCI Insight·Noelle YoungePatrick C Seed
Sep 10, 2020·Frontiers in Cellular and Infection Microbiology·Kehinde S OlaniyiIrene Mackraj
Dec 7, 2018·Nutrients·Anna PärttyMarko Kalliomäki
Jul 23, 2020·The Journal of Perinatal & Neonatal Nursing·Emily MalloyLisa Hanson
Feb 28, 2020·MSphere·Kevin R TheisNardhy Gomez-Lopez
Feb 20, 2020·Nutrients·Fiammetta PiersigilliOlivier Danhaive
May 10, 2020·Biological Reviews of the Cambridge Philosophical Society·Amar SarkarPhilip W J Burnet
Apr 1, 2021·Nature Reviews. Microbiology·Guanxiang Liang, Frederic D Bushman
Apr 5, 2021·Current Opinion in Virology·Frederic Bushman, Guanxiang Liang
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Mohi MiahMuzlifah Haniffa
Jul 29, 2021·Acta Paediatrica·Despina D BrianaAriadne Malamitsi-Puchner
Nov 2, 2021·The Journal of General Virology·Adam A WitneyBlair L Strang

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Methods Mentioned

BETA
PCR
dissections
dissection

Software Mentioned

SourceTracker
Greengenes
mothur
UCHIME
QIIME
PERMANOVA

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