Bacterial DNA sample preparation from whole blood using surface-modified Si pillar arrays

Analytical Chemistry
Kyu-Youn HwangJae-Chan Park

Abstract

A novel bacterial DNA sample preparation device for molecular diagnostics has been developed. On the basis of optimized conditions for bacterial adhesion, surface-modified silicon pillar arrays for bacterial cell capture were fabricated, and their ability to capture bacterial cells was demonstrated. The capture efficiency for bacterial cells such as Escherichia coli, Staphylococcus epidermidis, and Streptococcus mutans in buffer solution was over 75% with a flow rate of 400 microL/min. Moreover, the proposed method captured E. coli cells present in 50% whole blood effectively. The captured cells from whole blood were then in- situ lyzed on the surface of the microchip, and the eluted DNA was successfully amplified by qPCR. These results demonstrate that the full process of pathogen capture to DNA isolation from whole blood could be automated in a single microchip.

References

Oct 11, 1990·Nucleic Acids Research·B MercierC Mazurier
Dec 1, 1989·Experientia·C KrekelerJ Klein
Jul 1, 1983·Applied and Environmental Microbiology·D R AbsolomA W Neumann
Nov 1, 1984·Applied and Environmental Microbiology·H J BusscherJ Arends
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·F F BeckerP R Gascoyne
Nov 5, 1997·Applied and Environmental Microbiology·I G Wilson
Sep 1, 1996·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·J ChengP Wilding
Jan 5, 2000·Journal of Clinical Microbiology·W A Al-SoudP Râdström
Mar 4, 2000·Analytical Chemistry·X B WangP R Gascoyne
Dec 2, 2000·Journal of Clinical Microbiology·W Abu Al-Soud, P Rådström
Feb 7, 2001·Journal of Clinical Microbiology·W A Al-Soud, P Rådström
Apr 10, 2002·Analytical and Bioanalytical Chemistry·Ying HuangMarc Madou
Jul 12, 2005·Annual Review of Biomedical Engineering·Mehmet Toner, Daniel Irimia
Nov 18, 2005·Biosensors & Bioelectronics·Yoon-Kyoung ChoChristopher Ko
Mar 21, 2006·Colloids and Surfaces. B, Biointerfaces·C I PereniE Abel
Nov 23, 2006·Colloids and Surfaces. B, Biointerfaces·Fabien GaboriaudFabien Thomas
Oct 31, 2008·Talanta·Jan LichtenbergElisabeth Verpoorte
Jan 1, 1997·IEEE Transactions on Industry Applications·Peter R C GascoyneFrederick F Becker

❮ Previous
Next ❯

Citations

Jun 6, 2009·Analytical Biochemistry·S P MulvaneyL J Whitman
Feb 18, 2010·Lab on a Chip·Hun Joo LeeSoo Suk Lee
Oct 20, 2011·Lab on a Chip·Susann PodszunGerald A Urban
Apr 14, 2019·Annals of Biomedical Engineering·Alison Burklund, John X J Zhang
May 14, 2010·Analytical Chemistry·Arun AroraAndreas Manz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.