PMID: 9002617Dec 1, 1996Paper

Bacterial expression of an active class Ib chitinase from Castanea sativa cotyledons

Plant Molecular Biology
I AllonaC Aragoncillo

Abstract

Ch3, an endochitinase of 32 kDa present in Castanea sativa cotyledons, showed in vitro antifungal properties when assayed against Trichoderma viride. The characterization of a cDNA clone corresponding to this protein indicated that Ch3 is a class Ib endochitinase that is synthesized as a preprotein with a signal sequence preceding the mature polypeptide. Bacterial expression of mature Ch3 fused to the leader peptide of the periplasmic protein ompT resulted in active Ch3 enzyme. A plate assay was adapted for semi-quantitative determination of chitinase activity secreted from cultured bacteria, which should facilitate the identification of mutants with altered capacity to hydrolyse chitin.

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Citations

Oct 13, 2000·Biological Chemistry·Y PonathE Kombrink
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·J G BishopT Mitchell-Olds
Jul 29, 1998·The Journal of Allergy and Clinical Immunology·A Diaz-PeralesG Salcedo
Apr 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Dìaz-PeralesG Salcedo
Jul 9, 2014·Annual Review of Phytopathology·Jiatao Xie, Daohong Jiang
Feb 27, 2008·Journal of Agricultural and Food Chemistry·Devavratha H Rao, Lalitha R Gowda

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