PMID: 108245Mar 1, 1979

Bacterial fusion assayed by a prophage complementation test

Journal of Bacteriology
C Sanchez-Rivas, A J Garro


In previous studies of bacterial protoplast fusion, only the frequencies of cell wall regeneration and of bacterial recombination were determined. In this work the frequency of the heterozygous fusion products is measured by prophage complementation. Two multiply marked nonsuppressing strains of Bacillus subtilis, each lysogenic for a different Sus mutant of the phage phi 105, were induced by mitomycin C, protoplasted, fused, and, after dilution in hypertonic broth, incubated until plating with phi 105-sensitive indicator bacteria. When cell lysis was avoided, the frequency of the heterozygous fused cells could be determined from the number of infectious centers produced. The very high frequencies observed are in good agreement with those determined directly, with nonlysogenic strains, by electron microscopic examination of the fused protoplasts (C. Frehel, A. M. Lheritier, C. Sanchez-Rivas, and P. Schaeffer, J. Bacteriol. 137:1354--1361, 1979). Evidence is presented that fusion occurs in two steps, one polyethylene glycol dependent, the other energy requiring. The bacterial growth medium affects the ability of the protoplasts to fuse and to regenerate a cell wall. When experiments using different growth media were compared, an...Continue Reading


Jan 1, 1982·Molecular & General Genetics : MGG·C Sanchez-RivasP Schaeffer
Oct 1, 1988·Molecular & General Genetics : MGG·C Sanchez-RivasC Levi-Meyrueis
Jan 1, 1993·Archives of Microbiology·C P RubinsteinC Sanchez-Rivas

Related Concepts

Natto Bacteria
Cis-Trans Test
Recombination, Genetic

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