Bacterial Genome Containing Chimeric DNA-RNA Sequences

Journal of the American Chemical Society
Angad P MehtaPeter G Schultz

Abstract

Almost five decades ago Crick, Orgel, and others proposed the RNA world hypothesis. Subsequent studies have raised the possibility that RNA might be able to support both genotype and phenotype, and the function of RNA templates has been studied in terms of evolution, replication, and catalysis. Recently, we engineered strains of E. coli in which a large fraction of 2'-deoxycytidine in the genome is substituted with the modified base 5-hydroxymethyl-2'-deoxycytidine. We now report the generation of mutant strains derived from these engineered bacteria that show significant (∼40-50%) ribonucleotide content in their genome. We have begun to characterize the properties of these chimeric genomes and the corresponding strains to determine the circumstances under which E. coli can incorporate ribonucleotides into its genome and herein report our initial observations.

References

Jun 1, 1990·Journal of Bacteriology·G S BrushM J Bessman
Sep 24, 1987·Nature·S A Benner, A D Ellington
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·D Brown, L Gold
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C M Joyce
Sep 27, 2000·The Journal of Biological Chemistry·P H Patel, L A Loeb
Jul 12, 2002·Nature·Gerald F Joyce
Apr 1, 1955·Journal of Bacteriology·M LIEBE KELLENBERGER
Apr 5, 2007·Nature Protocols·Jill HerschlebDavid C Schwartz
May 10, 2007·Nature Reviews. Microbiology·Darius MoradpourCharles M Rice
Feb 26, 2009·Cell·Thomas R Cech
May 14, 2009·Chemical Communications : Chem Comm·Elizabeth O McCullum, John C Chaput
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stephanie A Nick McElhinnyThomas A Kunkel
Jul 9, 2010·Cold Spring Harbor Perspectives in Biology·Harry F Noller
Sep 1, 2009·Viruses·Ricardo FloresJosé-Antonio Daròs
May 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Christopher CozensPhilipp Holliger
Dec 19, 2012·DNA Repair·Anders R ClausenThomas A Kunkel
Jul 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nina Y YaoMike E O'Donnell
Jan 27, 2015·Nature Methods·Kyung Duk KohFrancesca Storici
Feb 11, 2015·Nucleic Acids Research·Liang XuDong Wang
Mar 31, 2015·Current Opinion in Microbiology·María A Sánchez-RomeroJosep Casadesús
Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Ryszard OlinskiDaniel Gackowski
May 24, 2016·Journal of the American Chemical Society·Angad P MehtaPeter G Schultz
Sep 22, 2016·Angewandte Chemie·Jesse V GavetteRamanarayanan Krishnamurthy
Nov 3, 2016·Journal of the American Chemical Society·Angad P MehtaPeter G Schultz
Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Paulina H WanrooijAndrei Chabes

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Citations

Sep 19, 2019·Nature Chemistry·Subhendu Bhowmik, Ramanarayanan Krishnamurthy
Aug 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peng NieHui Mei
Jul 28, 2020·Quarterly Reviews of Biophysics·Ali NikoomanzarJohn C Chaput
Feb 27, 2021·Journal of the American Chemical Society·Seohyun Chris KimJack W Szostak
Jul 2, 2021·Biochemistry·Mia A ShandellVirginia W Cornish

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