Bacterial incorporation of leucine into protein down to -20 degrees C with evidence for potential activity in sub-eutectic saline ice formations

Cryobiology
Karen JungeJody W Deming

Abstract

Direct evidence for metabolism in a variety of frozen environments has pushed temperature limits for bacterial activity to increasingly lower temperatures, so far to -20 degrees C. To date, the metabolic activities of marine psychrophilic bacteria, important components of sea-ice communities, have not been studied in laboratory culture, not in ice and not below -12 degrees C. We measured [3H]-leucine incorporation into macromolecules (further fractionated biochemically) by the marine psychrophilic bacterium Colwellia psychrerythraea strain 34H over a range of anticipated activity-permissive temperatures, from +13 to -20 degrees C, including expected negative controls at -80 and -196 degrees C. For incubation temperatures below -1 degrees C, the cell suspensions [all in artificial seawater (ASW)] were first quick-frozen in liquid nitrogen. We also examined the effect of added extracellular polymeric substances (EPS) on [3H]-leucine incorporation. Results showed that live cells of strain 34H incorporated substantial amounts of [3H]-leucine into TCA-precipitable material (primarily protein) down to -20 degrees C. At temperatures from -1 to -20 degrees C, rates were enhanced by EPS. No activity was detected in the killed controls f...Continue Reading

References

May 23, 1998·Critical Reviews in Biochemistry and Molecular Biology·N BurkhardtK H Nierhaus
Sep 30, 2000·Applied and Environmental Microbiology·E J CarpenterD G Capone
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·T Vajda
Mar 10, 2001·Nature·L J Rothschild, R L Mancinelli
Dec 10, 2002·Annual Review of Biophysics and Biomolecular Structure·R M DanielJ C Smith
Jan 9, 2004·Applied and Environmental Microbiology·Frédéric DumontPatrick Gervais
Mar 3, 2004·Microbial Ecology·J BreezeeJ T Staley
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·P Buford Price, Todd Sowers
Apr 20, 2004·Cryobiology·Gregory M FahyEric Zendejas
May 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Annette SieversRichard Wolfenden
Jan 8, 2005·Science·Oliver S WengerJay R Winkler
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Barbara A MethéClaire M Fraser
Mar 31, 1995·Science·C A Angell

❮ Previous
Next ❯

Citations

Sep 7, 2007·Microbial Ecology·Odd Gunnar BrakstadPer Johan Brandvik
Oct 21, 2009·Applied Microbiology and Biotechnology·Brent C Christner
May 16, 2013·Extremophiles : Life Under Extreme Conditions·R Eric Collins, Jody W Deming
Aug 30, 2013·The ISME Journal·Steven J TuortoLee J Kerkhof
Dec 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mickael VaïtilingomAnne-Marie Delort
Apr 5, 2007·Astrobiology·Nancy Y KiangRobert E Blankenship
Oct 28, 2006·Astrobiology·UNKNOWN MEPAG Special Regions-Science Analysis Group
Dec 9, 2008·Applied and Environmental Microbiology·Pierre Amato, Brent C Christner
Feb 5, 2009·Canadian Journal of Microbiology·P Buford Price
May 13, 2014·Nature Reviews. Microbiology·Janet K Jansson, Neslihan Taş
Dec 17, 2008·Environmental Microbiology·Pierre AmatoBrent C Christner
May 4, 2011·Environmental Microbiology·Corien Bakermans, Mark L Skidmore
Dec 1, 2011·Environmental Microbiology Reports·Corien Bakermans, Mark Skidmore
Dec 6, 2014·Extremophiles : Life Under Extreme Conditions·Charles V RiceRavindranth Garimella
Feb 25, 2015·Journal of Basic Microbiology·Mikako HashimotoHidetoshi Okuyama
Jul 22, 2014·FEMS Microbiology Ecology·Ido HatamBrian Lanoil
Apr 24, 2016·FEMS Microbiology Ecology·Mrinalini P NikradMax M Häggblom
Jun 13, 2018·FEMS Microbiology Reviews·Callum J D LeeJohn E Hallsworth
Jan 12, 2011·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Simon Conway Morris
Jul 17, 2012·Nature Reviews. Microbiology·Merry YouleS Craig Cary
Aug 15, 2017·Frontiers in Microbiology·Eva Garcia-Lopez, Cristina Cid
Aug 2, 2018·Frontiers in Physiology·Felizitas BajerskiJörg Overmann
Feb 17, 2021·Extremophiles : Life Under Extreme Conditions·Mackenzie K HaywardW Matthew Sattley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
P Buford Price
Applied and Environmental Microbiology
E M RivkinaD A Gilichinsky
Proceedings of the National Academy of Sciences of the United States of America
P Buford Price, Todd Sowers
© 2022 Meta ULC. All rights reserved