Bacterial lactoferrin receptors: insights from characterizing the Moraxella bovis receptors

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Rong-Hua Yu, Anthony B Schryvers

Abstract

Moraxella bovis is the causative agent of infectious conjunctivitis in cattle. Moraxella bovis isolates were shown to specifically bind bovine lactoferrin (bLf) and bovine transferrin (bTf) and to use these proteins as a source of iron to support the growth of iron-limited cells. Affinity isolation experiments with immobilized bTf yielded two proteins readily resolved by SDS-PAGE analysis, whereas only a single band of approximately 100 kDa was detected when immobilized bLf was used as the affinity ligand. Using a novel cloning strategy, regions containing the genes encoding the lactoferrin (Lf) and transferrin (Tf) receptor proteins were isolated and sequenced, demonstrating that they both consisted of two genes, with the tbpB or lbpB gene preceding the tbpA or lbpA gene. The cloned lbp genes were used to generate isogenic mutants deficient in lactoferrin binding protein A and (or) B, and the resulting strains were tested in growth and binding assays. The isogenic mutants were deficient in their use of bLf for growth and had substantially diminished bLf binding capability. The predicted amino acid sequence from the segment encoding Lf binding protein B revealed an internal amino acid homology suggesting it is a bi-lobed protei...Continue Reading

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Citations

Mar 23, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jeremy H Brock
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jessmi M L Ling, Anthony B Schryvers
Feb 16, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ari MorgenthauAnthony B Schryvers
Feb 16, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Elena ArutyunovaM Joanne Lemieux
Jun 1, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sarahí Luna-CastroMireya de la Garza
Jun 12, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Po-Wen ChenFang-Yi Chu
Feb 16, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Amanda J Beddek, Anthony B Schryvers
Jun 3, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Gabriela Cintia PostmaLeonardo Minatel
Aug 27, 2005·Australian Veterinary Journal·C S McConnel, J K House
Aug 9, 2003·Microbiology and Immunology·Tsutomu KakudaShinji Takai
Oct 19, 2004·Annual Review of Microbiology·Cécile Wandersman, Philippe Delepelaire
Aug 23, 2008·Biochimie·J L Flanagan, M D P Willcox
Jan 28, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·N OstanA B Schryvers
May 17, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Clement ChanAnthony B Schryvers
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Sep 8, 2016·Veterinary Research·Luisa Samaniego-BarrónMireya de la Garza
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Jun 29, 2004·Journal of Clinical Gastroenterology·Piera ValentiLucilla Seganti
May 30, 2021·The Veterinary Clinics of North America. Food Animal Practice·John Dustin LoyMichael L Clawson

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