Bacterial lipopolysaccharide induces porcine circovirus type 2 replication in swine alveolar macrophages

Veterinary Microbiology
Hui-Wen ChangChian-Ren Jeng

Abstract

Monocyte/macrophage lineage cells are major target cells of porcine circovirus type 2 (PCV2). From tissues of field pigs suffering from postweaning multisystemic wasting syndrome (PMWS), both intracytoplasmic and intranuclear PCV2 signals, including antigens and nucleic acid, were easily detected in the monocyte/macrophage lineage cells. However, there was a high incidence of intracytoplasmic PCV2-positive signals, but lack of intranuclear signals and PCV2 replication in these cells in vitro. Concurrent infection with bacteria and activation of immune system are suggested to promote viral replication. In this study, lipopolysaccharide (LPS) and phorbol-12-myristate-13-acetate (PMA) were used to stimulate PCV2-inoculated alveolar macrophages (AMs). A decrease in intracytoplasmic but increase in intranuclear PCV2-positive signals, including antigens and nucleic acid, were detected in LPS-treated PCV2-inoculated AMs, but not in PMA-treated cells. Additionally, the replication product corresponding to PCV2 spliced major capsid protein (Cap) mRNA and a significant elevation in PCV2 titer were demonstrated in the LPS-treated PCV2-inoculated AMs. The results imply that Gram-negative bacterial co-infection in PCV2-infected pigs may be ...Continue Reading

Citations

Dec 14, 2011·Animal Health Research Reviews·Yongming SangFrank Blecha
Dec 20, 2011·Virus Research·Laila Darwich, Enric Mateu
Jan 27, 2009·Veterinary Immunology and Immunopathology·Maria FortJoaquim Segalés
Jul 3, 2009·The Journal of General Virology·Sirje TimmuskCaroline Fossum
Jan 1, 2013·Annual Review of Animal Biosciences·Xiang-Jin Meng
Oct 18, 2018·Viruses·Ruifang WeiHans J Nauwynck
Apr 22, 2011·The Veterinary Record·N G A Woodger, O M Hosegood

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