Bacterial lipopolysaccharide induces rainbow trout myotube atrophy via Akt/FoxO1/Atrogin-1 signaling pathway

Acta Biochimica Et Biophysica Sinica
J E AedoJ A Valdés

Abstract

Lipopolysaccharide (LPS) is considered as a powerful inducer of muscle atrophy in higher vertebrates due to skeletal muscle cell recognition of the endotoxin and a consequent activation of catabolic signaling pathways. In contrast, there is no evidence of LPS directly inducing skeletal muscle atrophy in lower vertebrates, such as fish. For years it has been assumed that fish are resistant to LPS, mainly due to differences in the key features of toll-like receptor (TLR) signaling pathways when compared with mammals. In this study, we report that the stimulation of cultured rainbow trout (Oncorhynchus mykiss) myotubes with LPS (100 ng/ml) resulted in a transient decrease in the pAkt/Akt ratio, a subsequent reduction in the pFoxO1/FoxO1 ratio, and a significant increase in atrogin-1 transcript expression. Preincubation with polymyxin B, an LPS-neutralizing agent, and 740 Y-P, an agonist of p85-PI3K, blocked the effects of LPS. Additionally, LPS treatment induced an increase in protein ubiquitination and a reduction in myotube diameter, both of which are associated with muscular atrophy that is not observed under polymyxin B and 740 Y-P pretreatments. Finally, rainbow trout myotubes expressed the genes tlr1, tlr3, tlr5m, tlr8a1, tl...Continue Reading

References

Oct 29, 1998·Biochemical and Biophysical Research Communications·D DerossiP Doherty
Nov 7, 2001·Developmental and Comparative Immunology·J ZouC J Secombes
Aug 20, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert A FrostCharles H Lang
Jul 5, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert A FrostCharles H Lang
Jul 23, 2003·Bioinformatics·Perikles Simon
Oct 29, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert A FrostCharles H Lang
Dec 13, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Charles H LangRobert A Frost
Jul 4, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Katherine A JohansenKen Overturf
Feb 29, 2008·Cytokine·Yong-Chen LuPamela S Ohashi
Jul 8, 2008·Fish & Shellfish Immunology·P SwainS Dash
Sep 1, 2009·Experimental Cell Research·Steven T RussellMichael J Tisdale
Sep 10, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexander DoyleYi-Ping Li
Nov 19, 2010·Cell and Tissue Research·Jean Charles GabillardGilles Paboeuf
Dec 31, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yoryia VraskouJosep V Planas
Mar 19, 2011·Developmental and Comparative Immunology·Yniv Palti
Apr 15, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Iban SeiliezJean-Charles Gabillard
Jul 22, 2011·American Journal of Physiology. Cell Physiology·R TarabeesP T Loughna
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jan 2, 2013·American Journal of Physiology. Endocrinology and Metabolism·Nicolas J PillonAmira Klip
Nov 5, 2013·BMC Genomics·Nicholas J PooleySamuel A M Martin

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Citations

Aug 27, 2016·Journal of Cellular Biochemistry·Marlen B EspinozaJuan A Valdés
Apr 17, 2019·Journal of Muscle Research and Cell Motility·Jin WuShi-Tong Li
Jun 5, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Victoria CarrizoJuan Antonio Valdés

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