Bacterial lipopolysaccharide promotes profibrotic activation of intestinal fibroblasts

The British Journal of Surgery
J P BurkeP R O'Connell

Abstract

Fibroblasts play a critical role in intestinal wound healing. Lipopolysaccharide (LPS) is a cell wall component of commensal gut bacteria. The effects of LPS on intestinal fibroblast activation were characterized. Expression of the LPS receptor, toll-like receptor (TLR) 4, was assessed in cultured primary human intestinal fibroblasts using flow cytometry and confocal microscopy. Fibroblasts were treated with LPS and/or transforming growth factor (TGF) beta1. Nuclear factor kappaB (NFkappaB) pathway activation was assessed by inhibitory kappaBalpha (IkappaBalpha) degradation and NFkappaB promoter activity. Fibroblast contractility was measured using a fibroblast-populated collagen lattice. Smad-7, a negative regulator of TGF-beta1 signalling, and connective tissue growth factor (CTGF) expression were assessed using reverse transcriptase-polymerase chain reaction and western blot. The NFkappaB pathway was inhibited by IkappaBalpha transfection. TLR-4 was present on the surface of intestinal fibroblasts. LPS treatment of fibroblasts induced IkappaBalpha degradation, enhanced NFkappaB promoter activity and increased collagen contraction. Pretreatment with LPS (before TGF-beta1) significantly increased CTGF production relative to tr...Continue Reading

References

Sep 12, 1998·The Journal of Biological Chemistry·A IshisakiT Nishihara
Jan 15, 2000·Annals of Surgery·M C ReganP R O'Connell
Nov 30, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·J B PucilowskaP K Lund
Aug 24, 2001·The Journal of Clinical Investigation·G MonteleoneT T MacDonald
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Chunming DongPascal J Goldschmidt-Clermont
Jul 12, 2002·Kidney International·Annette KeilHarald D Rupprecht
Jun 14, 2003·The Cochrane Database of Systematic Reviews·K F GuenagaP Wille-Jørgensen
Jun 14, 2003·Gastroenterology·Jan-Michel OtteDaniel K Podolsky
Jul 28, 2004·Journal of Molecular and Cellular Cardiology·Gavin Y OuditPeter H Backx
Apr 20, 2005·Journal of Gastroenterology and Hepatology·Zhuobin ZhangYoshihide Fujiyama
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eoin P CumminsCormac T Taylor
Dec 13, 2006·The American Journal of Gastroenterology·John P BurkeP Ronan O'Connell
Dec 19, 2006·Gut·F RiederG Rogler
Jul 31, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ahmed M Elsharkawy, Derek A Mann
Oct 24, 2007·Nature Medicine·Ekihiro SekiRobert F Schwabe
Apr 11, 2008·American Journal of Physiology. Cell Physiology·Valérie HaydontMarie-Catherine Vozenin-Brotons
Jan 13, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kristen L W WaltonR Balfour Sartor

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Citations

Dec 14, 2012·Mucosal Immunology·B M J Owens, A Simmons
Apr 16, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Alexey V KostarnoyAlexandr L Gintsburg
Jun 21, 2013·Science Translational Medicine·Florian Rieder
Dec 25, 2013·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Peng LuDavid J Hackam
Apr 27, 2012·FEMS Immunology and Medical Microbiology·Haruko MiyazakiTetsuya Matsumoto
Jun 4, 2014·Inflammatory Bowel Diseases·Ilyssa O GordonFlorian Rieder
Nov 7, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Simone EbenerManuela Funke
Nov 7, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jill A PooleDebra J Romberger
Jul 30, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ellen J BeswickIrina V Pinchuk
Apr 7, 2020·Journal of Biomaterials Science. Polymer Edition·Ilyas InciHuseyin Avci
May 16, 2020·Journal of Digestive Diseases·Bo YangJie Wang
May 21, 2021·Journal of Digestive Diseases·Xiao Xuan LinRen Mao
Aug 3, 2021·Frontiers in Microbiology·Shukai ZhanZhirong Zeng

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