Bacterial lipopolysaccharide reduced intestinal barrier function and altered localization of 7H6 antigen in IEC-6 rat intestinal crypt cells

Journal of Cellular Physiology
H KimuraM Mori

Abstract

The intestinal epithelial barrier restricts the passage of potentially toxic substances into the systemic circulation and is considered to be mostly mediated by tight junctions, though the mechanisms involved in the regulation of intestinal tight junctions are not yet fully understood. In the present study, we examined whether bacterial lipopolysaccharide (LPS) altered the barrier function of tight junction and localization of tight junctional proteins, ZO-1 and 7H6 antigen, in IEC-6 intestinal cells. Administration of LPS to the basolateral surface of IEC-6 cells disrupted the barrier function and caused the disappearance of 7H6 antigen from the cell border, whereas LPS administered to the apical surface altered neither the barrier function nor the localization of 7H6 antigen in IEC-6 cells. On the other hand, the localization of ZO-1 was not influenced by these treatments of LPS. These results suggest that the interaction of LPS with the basolateral surface of intestinal epithelial cells disrupts the barrier function and 7H6 antigen take part in the maintenance of the barrier function in IEC-6 cells.

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Citations

Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T KawaguchiK Tanikawa
Dec 12, 2002·Progress in Biophysics and Molecular Biology·L González-MariscalB E Jaramillo
May 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T KawaguchiM Sata
Sep 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yoshihiko HirotaniKazuhiko Tanaka
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Oct 7, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Paola BrunDiego Martines
Jun 14, 2020·International Journal of Molecular Sciences·Basma AbdellatifAli Chaari
Mar 26, 2013·Archives of Animal Nutrition·Amal HalawaGerhard Breves
Aug 29, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Synia HaubIna Bergheim
Nov 14, 2019·Journal of Clinical Medicine·Dennis Gibson, Philip S Mehler
Apr 10, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tadanori YamadaMineko Fujimiya
Feb 17, 2018·ACS Biomaterials Science & Engineering·Hyesun HyunYoon Yeo

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