Bacterial lipopolysaccharide stimulates the thyrotropin-dependent thyroglobulin gene expression at the transcriptional level by involving the transcription factors thyroid transcription factor-1 and paired box domain transcription factor 8

Endocrinology
María Laura VélezAna María Masini-Repiso

Abstract

The bacterial lipopolysaccharide (LPS) is a biological activator that induces expression of multiple genes in several cell types. LPS has been proposed as an etiopathogenic agent in autoimmune diseases. However, whether LPS affects the expression of autoantigens has not been explored. Thyroglobulin (TG) is a key protein in thyroid hormonogenesis and one of the major thyroid autoantigens. This study aimed to analyze the action of LPS on TG gene expression in Fisher rat thyroid cell line FRTL-5 thyroid cells. We demonstrate that LPS increases the TSH-induced TG protein and mRNA level. Evidence that the effect of LPS is exerted at the transcriptional level was obtained by transfecting the minimal TG promoter. The C element of the TG promoter, which contains sequences for paired box domain transcription factor 8 (Pax8) and thyroid transcription factor (TTF)-1 binding, is essential for full TG promoter expression under TSH stimulation. The transcriptional activity of a construct containing five tandem repeats of the C site is increased by LPS, indicating a possible involvement of the C site in the LPS-induced TG gene transcription. We demonstrate that the TG promoter mutated at the Pax8 or TTF-1 binding element in the C site does no...Continue Reading

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Citations

Apr 17, 2010·Bulletin of Experimental Biology and Medicine·N V Yaglova
Aug 24, 2007·Thyroid : Official Journal of the American Thyroid Association·Leonardo Gabriel BazzaraAna María Masini-Repiso
Feb 14, 2016·Molecular and Cellular Endocrinology·Caroline Serrano-NascimentoMaria Tereza Nunes
Nov 28, 2015·Molecular and Cellular Endocrinology·María del Mar MontesinosAna María Masini-Repiso
Jun 20, 2015·International Journal of Endocrinology·Ana Paula Santin BertoniTania Weber Furlanetto
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Jan 23, 2016·American Journal of Physiology. Cell Physiology·Jamile Calil-SilveiraMaria Tereza Nunes
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Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity

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