Bacterial LPX motif-harboring virulence factors constitute a species-spanning family of cell-penetrating effectors

Cellular and Molecular Life Sciences : CMLS
Stefanie NorkowskiChristian Rüter

Abstract

Effector proteins are key virulence factors of pathogenic bacteria that target and subvert the functions of essential host defense mechanisms. Typically, these proteins are delivered into infected host cells via the type III secretion system (T3SS). Recently, however, several effector proteins have been found to enter host cells in a T3SS-independent manner thereby widening the potential range of these virulence factors. Prototypes of such bacteria-derived cell-penetrating effectors (CPEs) are the Yersinia enterocolitica-derived YopM as well as the Salmonella typhimurium effector SspH1. Here, we investigated specifically the group of bacterial LPX effector proteins comprising the Shigella IpaH proteins, which constitute a subtype of the leucine-rich repeat protein family and share significant homologies in sequence and structure. With particular emphasis on the Shigella-effector IpaH9.8, uptake into eukaryotic cell lines was shown. Recombinant IpaH9.8 (rIpaH9.8) is internalized via endocytic mechanisms and follows the endo-lysosomal pathway before escaping into the cytosol. The N-terminal alpha-helical domain of IpaH9.8 was identified as the protein transduction domain required for its CPE ability as well as for being able to d...Continue Reading

References

Mar 1, 1997·Infection and Immunity·J Nemeth, S C Straley
Jun 27, 2000·Bioinformatics·L J McGuffinD T Jones
Nov 30, 2000·The Journal of Peptide Research : Official Journal of the American Peptide Society·D J MitchellJ B Rothbard
Jun 22, 2001·The Journal of Biological Chemistry·T ToyotomeC Sasakawa
Dec 26, 2001·Current Opinion in Structural Biology·B Kobe, A V Kajava
Feb 15, 2002·Journal of Bacteriology·Briana M Young, Glenn M Young
Jul 10, 2003·Biochemical and Biophysical Research Communications·Per E G ThorénBengt Nordén
Jan 7, 2004·The Journal of Biological Chemistry·Rainer FischerRoland Brock
Feb 6, 2004·Molecular Microbiology·Sang Ho Lee, Jorge E Galán
Jun 14, 2005·Biochemical and Biophysical Research Communications·Jun OkudaChihiro Sasakawa
Oct 29, 2005·Chembiochem : a European Journal of Chemical Biology·Rainer FischerRoland Brock
Feb 17, 2006·Journal of Biochemical and Biophysical Methods·Fusinita van den Ent, Jan Löwe
Aug 29, 2006·Nature Reviews. Molecular Cell Biology·Juan S Bonifacino, Raul Rojas
Jul 31, 2007·Current Opinion in Immunology·Seema MattooJack E Dixon
Oct 16, 2007·Clinical Microbiology Reviews·Bryan CoburnB Brett Finlay
Nov 17, 2007·Cell Host & Microbe·John R RohdeClaude Parsot
Jun 20, 2008·Organic & Biomolecular Chemistry·Kelly M StewartShana O Kelley
Nov 11, 2008·Nature Structural & Molecular Biology·Yongqun ZhuFeng Shao
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
Mar 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cindy M QuezadaC Erec Stebbins
Mar 26, 2010·The Journal of Biological Chemistry·Joaquín Bernal-BayardFrancisco Ramos-Morales
Sep 14, 2010·FEBS Letters·Arefeh SeyedarabiRichard W Pickersgill
Nov 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir K VarkouhiHidde J Haisma
Mar 6, 2012·Analytical Biochemistry·William H Humphries, Christine K Payne
Jun 15, 2012·Nucleic Acids Research·Yana GofmanNir Ben-Tal
Jul 10, 2013·Cellular and Molecular Life Sciences : CMLS·Julia ScharnertChristian Rüter
Nov 23, 2013·FEMS Microbiology Letters·Christian Rüter, Philip R Hardwidge
May 8, 2014·Nature Reviews. Microbiology·Hiroshi AshidaChihiro Sasakawa
Sep 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shiho SuzukiChihiro Sasakawa
Nov 7, 2014·Nature Medicine·Doris PopovicIvan Dikic
Jan 3, 2015·Chemical Communications : Chem Comm·Ziqing QianDehua Pei

❮ Previous
Next ❯

Citations

Aug 24, 2018·Cellular Microbiology·Stefanie NorkowskiChristian Rüter
Mar 7, 2020·Pharmaceutics·Rebecca E Taylor, Maliha Zahid
Feb 19, 2021·Cellular Microbiology·Lena Hoang My LeRichard L Ferrero

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry
ubiquitination
pull-down
transfection
pull-downs
transmission electron microscopy
electron microscopy
fluorescence microscopy

Software Mentioned

MUSCLE
PyMOL
PSIPRED Protein Sequence Analysis Workbench server
MCPep
BioImageXD
JalView
PHYRE2 Protein Fold Recognition server

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.