PMID: 7536731Apr 1, 1995Paper

Bacterial luciferase as a reporter of circadian gene expression in cyanobacteria

Journal of Bacteriology
Y LiuC H Johnson

Abstract

To allow continuous monitoring of the circadian clock in cyanobacteria, we previously created a reporter strain (AMC149) of Synechococcus sp. strain PCC 7942 in which the promoter of the psbAI gene was fused to Vibrio harveyi luciferase structural genes (luxAB) and integrated into the chromosome. Northern (RNA) hybridization and immunoblot analyses were performed to examine changes in abundance of the luxAB mRNA, the native psbAI mRNA, and the luciferase protein to determine whether bioluminescence is an accurate reporter of psbAI promoter activity in AMC149. Under constant light conditions, the mRNA abundances of both luxAB and psbAI oscillated with a period of approximately 24 h for at least 2 days. The expression of these two genes following the same pattern: both mRNAs peaked in the subjective morning, and their troughs occurred near the end of the subjective night. The amount of luciferase protein also oscillated with a period of approximately 24 h, and the protein rhythm is in phase with the bioluminescence rhythm. The rhythm of the luciferase mRNA phase-leads the rhythms of luciferase protein and in vivo bioluminescence by several hours. Comparable results were obtained with a short-period mutant of AMC149. Together, the...Continue Reading

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Citations

Sep 1, 1996·Plant Molecular Biology·S JacobshagenC H Johnson
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y OuyangC H Johnson
Jan 30, 2002·Luminescence : the Journal of Biological and Chemical Luminescence·Lee F Greer, Aladar A Szalay
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Vikram VijayanErin K O'Shea
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mark L PaddockSusan S Golden
Feb 22, 2001·Annual Review of Physiology·C H Johnson
Aug 10, 2000·FEMS Microbiology Letters·J LorneV P Miao
Nov 5, 1999·Annual Review of Microbiology·C H Johnson, S S Golden
May 18, 2006·Plant & Cell Physiology·Masayuki Muramatsu, Yukako Hihara
Dec 4, 2012·Journal of Bacteriology·Vikram Vijayan, Erin K O'Shea
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Joseph S BoydSusan S Golden
May 15, 2002·FEMS Microbiology Letters·Kathryn A DurhamGeorge S Bullerjahn
Dec 22, 2007·Nature·Jeffrey R ChabotAlexander van Oudenaarden
Mar 15, 2005·Journal of Neurogenetics·Jason DeBruyneGregory M Cahill
Apr 19, 2008·Cold Spring Harbor Symposia on Quantitative Biology·S S Golden
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Susan S. GoldenTakao Kondo
Mar 24, 2004·Nature Reviews. Microbiology·Susan S Golden, Shannon R Canales
Jan 14, 2009·The Journal of Biological Chemistry·Zachary T Campbell, Thomas O Baldwin
Aug 28, 2009·The Journal of Biological Chemistry·Zachary T Campbell, Thomas O Baldwin
Jan 1, 2008·Journal of Bacteriology·Yoriko MurayamaTakao Kondo
Mar 29, 2001·Journal of Bacteriology·T Mori, C H Johnson
Oct 1, 1995·Current Opinion in Genetics & Development·M Rosbash
Nov 29, 1996·Molecular and Cellular Endocrinology·D A Carter, D Murphy
Apr 10, 2003·Fungal Genetics and Biology : FG & B·Louis W MorganDeborah Bell-Pedersen
Jan 24, 2008·BMC Microbiology·Attila KarsiMark L Lawrence
Jun 15, 1995·Genes & Development·Y LiuT Kondo

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