Bacterial-mediated synthesis of silver nanoparticles and their significant effect against pathogens

Environmental Science and Pollution Research International
Saira SaeedMuhammad Aqeel Ashraf

Abstract

Silver nanoparticles are potent antimicrobials and could be used as a promising alternative of conventional antibiotics. The aim of this study was to isolate bacteria from soil that have ability to produce AgNPs by secondary metabolite activity and their elucidation against human pathogens. These strains Escherichia coli, Exiguobacterium aurantiacumm, and Brevundimonas diminuta with NCBI accession number MF754138, MF754139, and MF754140 respectively were grown for secondary metabolite production. The nanoparticles were confirmed and characterized by UV-Vis spectroscopy and transmission electron microscopy. The optimization study was also carried out to obtain the maximum production of silver nanoparticles. Three parameters, temperature, pH, and AgNO3 concentration, were used to optimize the production of silver nanoparticles. Antimicrobial potential of these nanoparticles was addressed on the Muller-Hinton Agar, and their zones of inhibitions were measured. TEM analysis revealed the size and shape of the silver nanoparticles. All types of AgNPs were spherical in shape; their size range is from 5 to 50 nm. The findings of optimization study showed the maximum production of silver nanoparticles at the pH 9, temperature 37 °C, and...Continue Reading

References

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Citations

May 30, 2020·Environmental Science and Pollution Research International·Mona M ElsayedAyman Y El-Khateeb
Apr 20, 2021·Frontiers in Cellular and Infection Microbiology·Erika Kushikawa SaekiGerson Nakazato
May 4, 2021·Frontiers in Chemistry·Shubhrima GhoshSunil Kumar Khare
Jun 30, 2021·Applied Microbiology and Biotechnology·Giuseppe Gallo, Domenico Schillaci

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