Bacterial membrane binding and pore formation abilities of carbohydrate recognition domain of fish lectin

Developmental and Comparative Immunology
Abirami ArasuJesu Arockiaraj

Abstract

Antimicrobial peptides (AMPs) are innate molecules that are found in a wide variety of species ranging from bacteria to humans. In recent years, excessive usage of antibiotics resulted in development of multi-drug resistant pathogens which made researchers to focus on AMPs as potential substitute for antibiotics. Lily type mannose-binding lectin is an extended super-family of structurally and evolutionarily related sugar binding proteins. These lectins are well-known AMPs which play important roles in fish defense mechanism. Here, we report a full-length lily type lectin-2 (LTL-2) identified from the cDNA library of striped murrel, Channa striatus (Cs). CsLTL-2 protein contained B-lectin domain along with three carbohydrate binding sites which is a prominent characteristic functional feature of LTL. The mRNA transcripts of CsLTL-2 were predominantly expressed in gills and considerably up-regulated upon infection with fungus (Aphanomyces invadans) and bacteria (Aeromonas hydrophila). To evaluate the antimicrobial activity of the carbohydrate binding region of CsLTL-2, the region was synthesized (QP13) and its bactericidal activity was analyzed. In addition, QP13 was labeled with fluorescein isothiocyanate (FITC) and its binding ...Continue Reading

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Citations

Jul 28, 2018·Journal of Applied Microbiology·Luana C B B CoelhoM T Dos Santos Correia
Jul 28, 2020·Cell Biology International·Anbazahan SannasimuthuJesu Arockiaraj
Nov 30, 2018·Frontiers in Microbiology·Maarten G K GhequireRené De Mot
Jul 6, 2020·The Protein Journal·Prabha NagaramJesu Arockiaraj
Jan 20, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Stefi V RajuJesu Arockiaraj
May 20, 2021·Saudi Journal of Biological Sciences·Dengke LiuPing Zhao

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