Bacterial Memory of Persisters: Bacterial Persister Cells Can Retain Their Phenotype for Days or Weeks After Withdrawal From Colony-Biofilm Culture

Frontiers in Microbiology
Saki MiyaueSumio Maeda

Abstract

Persister cells, or persisters, are a specific subpopulation of bacterial cells that have acquired temporary antibiotic-resistant phenotypes. In this study, we showed that Escherichia coli produces many more persister cells in colony-biofilm culture than in the usual liquid culture and that these persisters can be maintained in higher numbers than those from liquid culture for up to 4 weeks at 37°C in a fresh, nutrient-rich, antibiotic-containing medium, even after complete withdrawal from the colony-biofilm culture. This suggests the presence of a long-retention effect, or "memory effect", in the persister cell state of E. coli cells. We also discovered that such increases in persisters during colony-biofilm culture and their memory effects are common, to a greater or lesser degree, in other bacterial species. This is true not only for gram-negative bacteria (Acinetobacter and Salmonella) but also for gram-positive bacteria (Staphylococcus and Bacillus). This is the first report to suggest the presence of a common memory mechanism for the persister cell state, which is inscribed during colony-biofilm culture, in a wide variety of bacteria.

References

Jan 1, 1991·Annual Review of Genetics·G G Wilson, N E Murray
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·K Lewis
Apr 16, 2002·Molecular Microbiology·Ruud JansenLeo M Schouls
Jan 1, 1953·Cold Spring Harbor Symposia on Quantitative Biology·S E LURIA
Sep 15, 2004·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·John C CarmenWilliam G Pitt
Jan 27, 2006·FEMS Microbiology Letters·Sumio MaedaShiho Kato
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josep Casadesús, David Low
Jan 31, 2009·Journal of Biosciences·R Jayaraman
Jan 9, 2010·Science·Philippe Horvath, Rodolphe Barrangou
Jun 10, 2010·Annual Review of Microbiology·Kim Lewis
Sep 20, 2011·Nature Reviews. Microbiology·Yoshihiro Yamaguchi, Masayori Inouye
Jan 8, 2013·FEMS Microbiology Letters·Christopher F Schuster, Ralph Bertram
Sep 17, 2013·Applied and Environmental Microbiology·Thomas K WoodBrian W Kwan
Mar 14, 2014·Frontiers in Microbiology·Stephanie M AmatoMark P Brynildsen
Mar 29, 2014·PloS One·Stephanie M Amato, Mark P Brynildsen
Jul 18, 2015·Microbiology Spectrum·Philip S Stewart
Aug 12, 2016·Nature Reviews. Microbiology·Hans-Curt FlemmingStaffan Kjelleberg
Dec 4, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Joran Elie MichielsJan Michiels
Dec 17, 2016·Science·Alexander HarmsKenn Gerdes
Mar 24, 2017·FEMS Microbiology Reviews·Bram Van den BerghJan Michiels
May 23, 2017·Nature Reviews. Microbiology·Robert A FisherSophie Helaine
Jul 16, 2017·European Biophysics Journal : EBJ·Tahmina RahmanDeclan A Doyle
Mar 27, 2018·Microbiology and Immunology·Yuto KawaiSatoshi Tsuneda

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Citations

Jul 16, 2019·FEMS Microbiology Ecology·Paula JorgeMaria Olívia Pereira
Nov 28, 2019·International Journal of Molecular Sciences·Mariana Adina MaticaVasile Ostafe
Aug 27, 2019·Frontiers in Molecular Biosciences·Kshitiz Gupta, Sanjay Chhibber
Oct 9, 2020·Critical Reviews in Microbiology·Chaminda J SeneviratneGordon Ramage
Aug 30, 2019·Heliyon·Gebreselema GebreyohannesDesta Berhe Sbhatu
Jan 21, 2021·Future Microbiology·Bárbara Ellen Santos CarvalhaisKênia Valéria Dos Santos
Feb 14, 2021·Antibiotics·Gracja Topka-BieleckaAlicja Węgrzyn
Oct 29, 2020·BMC Molecular and Cell Biology·Cyrus AlexanderTushar Kant Beuria
Jan 13, 2021·NPJ Biofilms and Microbiomes·Eleftheria TrampariMark A Webber
Dec 3, 2020·International Journal of Molecular Sciences·Shiqi LiuSebastian A J Zaat
Nov 25, 2020·Antibiotics·Christian Emmanuel MahavyTsiry Rasamiravaka
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Harinash RaoPei Pei Chong
Jun 2, 2021·World Journal of Microbiology & Biotechnology·Erika SuzukiSumio Maeda

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Methods Mentioned

BETA
restriction modification

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