Bacterial molecular epidemiology based on a nonradioactive probe complementary to ribosomal RNA.

Research in Microbiology
M Altwegg, L W Mayer

Abstract

The use of plasmid pKK3535 (a pBR322-derived plasmid containing a ribosomal RNA operon) is described for determining rRNA gene restriction patterns. The plasmid allowed the use of both radioactive as well as nonradioactive procedures and provided features that are necessary for the inclusion of internal and/or external standards that facilitate comparison of strains. The patterns generated are identical to those obtained by hybridization with 32P-labelled rRNA.

References

Sep 1, 1986·Annales De L'Institut Pasteur. Microbiology·F Grimont, P A Grimont
Feb 1, 1988·The Journal of Infectious Diseases·T L StullT D Edlind
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P R LangerD C Ward

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Citations

Jan 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M AltweggM Rohrbach
Jul 1, 1990·Research in Microbiology·S KoblaviP A Grimont
Oct 10, 1998·Research in Microbiology·B RegnaultP A Grimont
Aug 1, 2000·Avian Pathology : Journal of the W.V.P.A·P J Blackall, J K Miflin
Oct 2, 1998·Revista Do Instituto De Medicina Tropical De São Paulo·C T SacchiL W Mayer
Jul 1, 1995·Revista Do Instituto De Medicina Tropical De São Paulo·C T SacchiA Sperb
Jul 1, 1994·Clinical Microbiology Reviews·E H BingenJ Elion
Jun 1, 1992·Applied and Environmental Microbiology·N P MoyerM Altwegg
Jul 1, 1994·Journal of Clinical Microbiology·C T SacchiC E Melles

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