Bacterial population dynamics and faecal short-chain fatty acid (SCFA) concentrations in healthy humans

The British Journal of Nutrition
Alexandra L McOristKerry Nyland

Abstract

Fermentation products, SCFA, particularly butyrate, are considered a sign of 'good' bowel health but the influence of bacterial population composition and diet on inter-individual difference in metabolites and colonic health is poorly understood. Faecal specimens were collected weekly from eight healthy human volunteers over 12 weeks. Dietary intake was self-reported and ten macronutrient factors were analysed at selected weekly periods. Faecal weight, pH and moisture were recorded, and SCFA concentrations were measured in all samples. From each specimen, DNA was prepared and eubacterial 16S rRNA gene PCR performed. Bacterial population profiles were captured by denaturing gradient gel electrophoresis (DGGE) of PCR products, and multivariate statistical analysis was performed. Faecal weight, pH and moisture varied widely within and between individuals. Average total SCFA concentrations over 12 weeks ranged from 36.9 to 144.4 mmol/kg in 48 h specimens and faecal butyrate concentrations ranged from 1.8 to 48.5 mmol/kg. Two individuals with butyrate concentrations below 10 mmol/kg were considered to be 'low butyrate types' and may represent an at-risk population for bowel health. Dietary fat, sugar and carbohydrate showed weak cor...Continue Reading

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Citations

Mar 2, 2011·Canadian Journal of Microbiology·Clarissa Schwab, Michael Gänzle
Jan 12, 2011·BMC Gastroenterology·Abdul Q KhanBernard M Corfe
Apr 27, 2012·Digestive Diseases and Sciences·Lv WangMichael Allan Conlon
Dec 25, 2008·Cell Biochemistry and Function·Marco A R VinoloRui Curi
Apr 29, 2015·Arthritis & Rheumatology·Angélica T VieiraCharles R Mackay
Mar 25, 2015·Free Radical Biology & Medicine·Gema Pereira-CaroMary Ann Augustin
Sep 1, 2015·World Journal of Biological Chemistry·Sabrina Chriett, Luciano Pirola
Jul 15, 2015·Nutrition Research Reviews·Kristin A VerbekeKieran M Tuohy
Jan 1, 2016·Molecular Pharmacology·Daniele BologniniCatherine E Moss
Mar 25, 2011·The Journal of Nutrition·Alexandra L McOristMichael A Conlon
Sep 24, 2011·The American Journal of Clinical Nutrition·Julie M ClarkeLynne Cobiac
May 10, 2020·The Proceedings of the Nutrition Society·A DagbasiG Frost
Sep 18, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Natalia ZarateS Mark Scott
May 30, 2017·Journal of Atherosclerosis and Thrombosis·Hideo OhiraYoshio Fujioka

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