Bacterial products primarily mediate fibroblast inhibition in biomaterial infection

The Journal of Surgical Research
P K HenkeJ C Peyton

Abstract

The stimulation of fibroblast growth is essential for the normal healing and tissue integration of biomaterials. The local elevation of proinflammatory mediators in infected perigraft fluid (PGF) may inhibit this growth. We sought to determine whether infected PGF inhibited fibroblast growth, and, if so, whether this was primarily dependent on the biomaterial, bacteria, or host. In vivo Dacron or expandable polytetra-fluoroethylene (ePTFE) grafts, sterile or colonized with slime-producing (RP-62A, viable or formalin-killed) or nonslime-producing (RP-62NA) Staphylococcus epidermidis (1 x 10(7) CFU/cm2), were implanted in Swiss Webster mice, and the PGF was harvested at 7 and 28 days. Antibodies to tumor necrosis factor alpha, interleukin 1 alpha, interferon gamma (7 micrograms/day), and indomethacin (50 micrograms/day) were administered by microinfusion pumps for 7 days and the PGF was harvested. Inhibition of the proinflammatory mediators was confirmed by enzyme-linked immunosorbant assay. The nontreated, heat-treated, or trypsin-digested in vivo PGF was incubated with an in vitro [3H]thymidine murine fibroblast (ATCC CCL-12) proliferation assay. Fibroblast inhibition was significant at 7 and 28 days with infected PGF incubatio...Continue Reading

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Citations

Oct 30, 2007·European Journal of Obstetrics, Gynecology, and Reproductive Biology·L BoulangerM Cosson
Apr 9, 2002·Journal of Chemotherapy·J M SchierholzG Pulverer
Sep 25, 2001·The Journal of Hospital Infection·J M Schierholz, J Beuth
May 27, 2006·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·A YasimY Ergun
Jan 10, 2008·International Urogynecology Journal and Pelvic Floor Dysfunction·Loïc BoulangerMichel Cosson
Feb 10, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·E M EddsK R Brittian

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