Bacterial RNA polymerase caps RNA with various cofactors and cell wall precursors

Nucleic Acids Research
Christina Julius, Yulia Yuzenkova

Abstract

Bacterial RNA polymerase is able to initiate transcription with adenosine-containing cofactor NAD+, which was proposed to result in a portion of cellular RNAs being 'capped' at the 5' end with NAD+, reminiscent of eukaryotic cap. Here we show that, apart from NAD+, another adenosine-containing cofactor FAD and highly abundant uridine-containing cell wall precursors, UDP-Glucose and UDP-N-acetylglucosamine are efficiently used to initiate transcription in vitro. We show that the affinity to NAD+ and UDP-containing factors during initiation is much lower than their cellular concentrations, and that initiation with them stimulates promoter escape. Efficiency of initiation with NAD+, but not with UDP-containing factors, is affected by amino acids of the Rifampicin-binding pocket, suggesting altered RNA capping in Rifampicin-resistant strains. However, relative affinity to NAD+ does not depend on the -1 base of the template strand, as was suggested earlier. We show that incorporation of mature cell wall precursor, UDP-MurNAc-pentapeptide, is inhibited by region 3.2 of σ subunit, possibly preventing targeting of RNA to the membrane. Overall, our in vitro results propose a wide repertoire of potential bacterial RNA capping molecules, ...Continue Reading

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Citations

Apr 7, 2018·Transcription·Christina JuliusYulia Yuzenkova
Jul 27, 2018·RNA·Ewa Grudzien-NogalskaMegerditch Kiledjian
Oct 3, 2018·Wiley Interdisciplinary Reviews. RNA·Nikita VasilyevAlexander Serganov
Oct 26, 2018·Wiley Interdisciplinary Reviews. RNA·Christina Julius, Yulia Yuzenkova
May 7, 2020·Nucleic Acids Research·Selom K DoamekporLiang Tong
Aug 22, 2020·FEMS Microbiology Letters·D Levi CraftPeter Setlow
May 19, 2019·Nature Chemical Biology·Ewa Grudzien-NogalskaMegerditch Kiledjian
Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Daniel J Luciano, Joel G Belasco
Apr 6, 2019·Nucleic Acids Research·Aleksandra KwasnikJoanna Kufel
Sep 11, 2019·Nucleic Acids Research·Jin WangPeter C Dedon
Jul 23, 2020·Nature Metabolism·Elena KatsyubaJohan Auwerx
Nov 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anais Depaix, Joanna Kowalska
Apr 18, 2020·Wiley Interdisciplinary Reviews. RNA·Phillip J McCownJessica A Brown
Oct 6, 2020·Frontiers in Microbiology·Diego A Vargas-Blanco, Scarlet S Shell
Dec 11, 2020·Wiley Interdisciplinary Reviews. RNA·Jerry PelletierNahum Sonenberg
May 22, 2021·Royal Society Open Science·Jana WiedermannováYulia Yuzenkova
Jun 20, 2018·Microbiology Spectrum·Katharina Höfer, Andres Jäschke
Jun 15, 2021·Advanced Biology·Maik SchauerteKatharina Höfer
Jul 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kyle S SkalenkoBryce E Nickels
Sep 23, 2021·Nucleic Acids Research·Jana Wiedermannová, Libor Krásný

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Software Mentioned

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