Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays

Nature Biotechnology
A de SaizieuJ Mous

Abstract

We have used high-density oligonucleotide probe arrays (chips) for bacterial transcript imaging. We designed a chip containing probes representing 106 Hemophilus influenzae genes and 100 Streptococcus pneumoniae genes. The apparent lack of polyadenylated transcripts excludes enrichment of mRNA by affinity purification and we thus used total, chemically biotinylated RNA as hybridization probe. We show that hybridization of Streptococcus RNA to a chip allows simultaneous quantification of the transcript levels. The sensitivity was found to be in the range of one to five transcripts per cell. The quantitative chip results were in good agreement with conventional Northern blot analysis of selected genes. This technology allows simultaneous and quantitative measurement of the transcriptional activity of entire bacterial genomes on a single oligonucleotide probe array.

References

Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·L S HåvarsteinD A Morrison
Feb 1, 1994·Infection and Immunity·G Plum, J E Clark-Curtiss
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·A C PeaseS P Fodor
Apr 1, 1993·Journal of Bacteriology·S E ChuangF R Blattner
May 1, 1997·Nucleic Acids Research·R FislageH Oberender
Feb 1, 1997·Molecular Microbiology·Q ChengH R Masure
Dec 1, 1996·Nature Biotechnology·D J LockhartE L Brown
Oct 1, 1997·Journal of Applied Microbiology·J-P ClaverysG Alloing

❮ Previous
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Citations

Jul 17, 2008·Comparative and Functional Genomics·Tahir R AliPaul R Langford
Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Kazuhiro SuzukiHidetoshi Yamanaka
Jun 22, 2000·Analytical Biochemistry·M Lee, D R Walt
Nov 30, 2000·Current Infectious Disease Reports·D Knowles, G Patou
Apr 13, 2001·Trends in Biotechnology·G Grandi
Sep 2, 2003·Journal of Biotechnology·Volker F Wendisch
Feb 27, 2001·Brain Research. Molecular Brain Research·M DoyuK Kato
Oct 12, 2001·Journal of Physiology, Paris·S SzaboH Matsumoto
Mar 14, 2000·Current Opinion in Immunology·I D Manger, D A Relman
Dec 5, 2000·Current Opinion in Biotechnology·L E Alksne, S J Projan
Aug 16, 2002·Current Opinion in Biotechnology·Jizhong Zhou, Dorothea K Thompson
Jan 16, 1999·Current Opinion in Biotechnology·N J Saunders, E R Moxon
Jun 11, 1999·Current Opinion in Biotechnology·B J MacGregor
Jun 12, 2002·Current Opinion in Microbiology·John I GlassGregory T Robertson
Dec 1, 2001·Trends in Microbiology·D McDevitt, M Rosenberg
Mar 20, 1999·Genetic Analysis : Biomolecular Engineering·R J SapolskyJ B Fan
Aug 6, 1998·Current Opinion in Chemical Biology·S C Case-GreenE M Southern
Sep 16, 1998·Current Opinion in Chemical Biology·M B Schmid
Jun 14, 2000·Current Opinion in Microbiology·C A HarringtonJ Retief
May 2, 2002·Journal of Hematotherapy & Stem Cell Research·Timo GaiserKarin Lange
Jul 25, 2000·Nucleic Acids Research·A KumarZ Liang
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H Smith
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·I Hautefort, J C Hinton
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R Moxon, C Tang
Oct 19, 1999·Microbiology and Immunology·I H ChoiD K Rhee
Feb 7, 2001·Applied and Environmental Microbiology·S G BavykinA D Mirzabekov
Jul 27, 2001·Applied and Environmental Microbiology·B GustM Redenbach
Feb 7, 2003·Applied and Environmental Microbiology·Philip DennisRoberta Fulthorpe

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