Bacterial viability in faecal transplants: Which bacteria survive?

EBioMedicine
Lito E PapanicolasGeraint B Rogers

Abstract

The therapeutic potential of faecal microbiota transplantation (FMT) is under investigation for a range of inflammatory conditions. While mechanisms of benefit are poorly understood, most models rely on the viability of transplanted microbes. We hypothesised that protocols commonly used in the preparation of faecal transplants will substantially reduce the number, diversity and functional potential of viable microbes. Stools from eight screened donors were processed under strict anaerobic conditions, in ambient air, and freeze-thawed. Propidium monoazide (PMA) sample treatment was combined with quantitative PCR, 16S rRNA gene amplicon sequencing and short-chain fatty acid (SCFA) analysis to define the viable microbiota composition and functional potential. Approximately 50% of bacterial content of stool processed immediately under strict anaerobic conditions was non-viable. Homogenisation in ambient air or freeze-thaw reduced viability to 19% and 23% respectively. Processing of samples in ambient air resulted in up to 12-fold reductions in the abundance of important commensal taxa, including the highly butyrogenic species Faecalibacterium prausnitzii, Subdoligranulum variable, and Eubacterium hallii. The adverse impact of atmos...Continue Reading

Citations

Jan 16, 2020·Animals : an Open Access Journal From MDPI·Ivan Rychlik
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Oct 23, 2019·Signal Transduction and Targeted Therapy·Ziying ZhangYongguang Tao
Feb 5, 2021·IScience·Jocelyn M Choo, Geraint B Rogers
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Jun 16, 2021··Jocelyn M. Choo, Geraint B. Rogers

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Datasets Mentioned

BETA
PRJNA491383

Methods Mentioned

BETA
amplicon sequencing
PMA
nucleic acid sequencing
PCR
amplicon

Software Mentioned

ANO
Quantitative Insights in to Microbial Ecology ( QIIME )
GraphPad Prism
QIIME

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