Bactericidal in-vitro activity of beta-lactams and beta-lactamase inhibitors, alone or associated, against clinical strains of Acinetobacter baumannii: effect of combination with aminoglycosides

The Journal of Antimicrobial Chemotherapy
M L Joly-GuillouE Bergogne-Bérézin

Abstract

Five Acinetobacter baumannii strains of various phenotypes were selected on the basis of the results of a national survey in France in 1991. beta-Lactamases in Acinetobacter isolates were characterized by isoelectrofocusing. We carried out a 24 h time-kill study to assess the bactericidal effect of antibiotic alone or in combination against A. baumannii strains. The initial inoculum was 10(6) cfu/mL. Antibiotics were tested at MIC x 2 when an antibiotic was tested alone and at the MIC for each combination including ticarcillin, piperacillin, ceftazidime or imipenem with or without amikacin or netilmicin and/or beta-lactamase inhibitors. Concentration of inhibitors were: 2 mg/L for clavulanic acid, 4 mg/L for tazobactam and 8 mg/L for sulbactam. Sulbactam and tazobactam showed an intrinsic in-vitro activity against strains susceptible to ticarcillin. A complete killing at 24 h was observed when beta-lactams were combined with amikacin in comparison with antibiotics alone. Synergy was lost when the strain presented a low resistance level to beta-lactams or aminoglycosides except for ticarcillin. Combinations of sulbactam with ticarcillin showed the best bactericidal activity against strains multiresistant to beta-lactams.

Citations

Jan 1, 2003·International Journal of Antimicrobial Agents·Anna S LevinSilvia F Costa
Jul 11, 2013·The Journal of Antimicrobial Chemotherapy·Lenka KrizovaAlexandr Nemec
Nov 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·P Gerner-Smidt, I Tjernberg
Jul 16, 2008·Clinical Microbiology Reviews·Anton Y PelegDavid L Paterson
Feb 8, 2005·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Joel N MaslowMax Lataillade
Jun 24, 2008·International Journal of Antimicrobial Agents·Helen GiamarellouKyriaki Kanellakopoulou
Jul 25, 2003·Expert Opinion on Pharmacotherapy·Anna S Levin
Jul 28, 2005·The Journal of Antimicrobial Chemotherapy·Josée E Roy-LeonD William Cameron

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