Bacteriocin activity of Lactobacillus brevis and Lactobacillus paracasei ssp. paracasei

Journal of Medical Microbiology
Ramona IseppiC Sabia

Abstract

Biochemical and molecular methods were used to identify 100 lactobacilli isolated from rectal swabs. Among these, L. paracasei ssp. paracasei LP5 and L. brevis LP9 showed significant antibacterial activity against S. agalactiae and L. monocytogenes. Accordingly, characterization of their bacteriocins, BacLP5 and BacLP9, was conducted to obtain information on their kinetic production, sensitivity to chemico-physical parameters and molecular weight. To investigate the possible use of the two Lactobacillus strains as probiotics, their gastrointestinal resistance, cellular adhesiveness and sensitivity to antibiotics were also studied. The obtained data show that BacLP5 and BacLP9 most likely belong to class II bacteriocins and both have a molecular weight of approximately 3 kDa. The production of BacLP5 and BacLP9 started after 4 h (40 and 80 AU ml-1), respectively. Both of the Lactobacillus strains survived gastric and intestinal juices well and showed adhesive capability on HEp-2 cells. Due to their peculiar antimicrobial characteristics, L. paracasei ssp. paracasei LP5 and L. brevis LP9 are suitable for use in the treatment of vaginal disorders, through both oral and transvaginal administration.

References

Mar 1, 1992·Annals of Internal Medicine·E HiltonM T Borenstein
Aug 1, 1970·Applied Microbiology·D A Kékessy, J D Piguet
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Apr 1, 1993·Epidemiology and Infection·P A Grey, S M Kirov
Oct 1, 1996·Antonie van Leeuwenhoek·I F NesH Holo
Feb 2, 2000·World Journal of Urology·G W Tannock
Feb 13, 2001·Journal of Biotechnology·M SaarelaT Mattila-Sandholm
Feb 15, 2001·FEMS Immunology and Medical Microbiology·G ReidB Henning
Oct 24, 2002·Journal of Applied Microbiology·P MastromarinoD Matteuzzi
Jul 5, 2005·The Journal of Infectious Diseases·May A D AntonioSharon L Hillier
Feb 26, 2009·Letters in Applied Microbiology·K A RyanP W O'Toole
Jan 1, 2008·Interdisciplinary Perspectives on Infectious Diseases·Sarah CribbyGregor Reid
Apr 14, 2009·Seminars in Fetal & Neonatal Medicine·Jeff M Denney, Jennifer F Culhane
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jacques RavelLarry J Forney
Apr 26, 2011·American Journal of Obstetrics and Gynecology·Jenifer E Allsworth, Jeffrey F Peipert
Feb 12, 2013·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Goran VujicVedrana Kuzmic Vrbanovic
Feb 14, 2014·Journal of Medical Microbiology·Priscilla Romina De GregorioMaría Elena Fátima Nader-Macías
Aug 19, 2015·Microbial Ecology in Health and Disease·Jean M MacklaimGregor Reid
Feb 13, 2016·Jundishapur Journal of Microbiology·Abolfazl DavoodabadiMaryam Tajabadi Ebrahimi
Feb 21, 2016·Applied and Environmental Microbiology·Nemanja MirkovicMilan Kojic
May 18, 2016·FEMS Microbiology Letters·Thibault AllainLuis G Bermúdez-Humarán
Jan 23, 2018·Journal of Menopausal Medicine·Jun-Mo Kim, Yoo Jin Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.