Bacteriocin-Producing Lactic Acid Bacteria Isolated from Mangrove Forests in Southern Thailand as Potential Bio-Control Agents: Purification and Characterization of Bacteriocin Produced by Lactococcus lactis subsp. lactis KT2W2L

Probiotics and Antimicrobial Proteins
Noraphat HwanhlemJean-Marc Chobert

Abstract

The aim of this work was to purify and characterize the bacteriocin produced by Lactococcus lactis subsp. lactis KT2W2L previously isolated from mangrove forests in southern Thailand, in order to evaluate its potential as new food protective agent. The active peptide from the cell-free supernatant of this strain was purified in 4 steps: (1) precipitation with 70 % saturated ammonium sulfate, (2) elution on a reversed-phase cartridge using different concentrations of acetonitrile, (3) cation-exchange chromatography and (4) final purification by reversed-phase HPLC on a C8 column. The molecular mass of 3,329.5254 Da of the purified bacteriocin, determined by mass spectrometry, is nearly identical to that of peptide nisin Z. The activity of the purified bacteriocin was unaffected by pH (2.0-10.0), thermostable but was sensitive to proteolytic enzymes. The bacteriocin activity was stable after 8 weeks of storage at -20 °C and 7 weeks of storage at 4 °C, but decreased after 3 weeks of storage at 37 °C. It was stable when incubated for 1 month at 4 °C in 0-30 % NaCl. Inhibitory spectrum of this bacteriocin showed a wide range of activity against similar bacterial strains, food-spoilage and food-borne pathogens. L. lactis subsp. lacti...Continue Reading

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Citations

Aug 15, 2014·Probiotics and Antimicrobial Proteins·Ana Beatriz Jeronymo-CenevivaAna Lúcia B Penna
Sep 28, 2017·Applied Biochemistry and Biotechnology·Qianwen XiZhijiang Zhou
Jul 25, 2020·Scientific Reports·Pamela Oliveira de Souza de AzevedoRicardo Pinheiro de Souza Oliveira
Mar 8, 2019·Probiotics and Antimicrobial Proteins·Graciele Daiana FunckÂngela Maria Fiorentini
Mar 8, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Pamela O S de AzevedoRicardo P S Oliveira
Jun 19, 2021·Protein and Peptide Letters·Vaithiyanathan SuganthiMohanasrinivasan Vaithilingam

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