Bacteriocinogenic and virulence potential of Enterococcus isolates obtained from raw milk and cheese

Journal of Applied Microbiology
P M MoraesL A Nero

Abstract

To provide molecular and phenotypical characterization of Enterococcus isolates obtained from raw milk and cheese, regarding their bacteriocinogenic and virulence activity. Forty-three bacteriocinogenic enterococci isolates were identified by 16s rDNA, fingerprinted by RAPD-PCR analysis and tested by PCR for the presence of genes for lantibiotics (lanM, lanB and lanC) and enterocins (entA, entB, entP, entL50AB and entAS48) and by phenotypical methods for bacteriocin production and inhibitory spectrum. Also, the virulence of the isolates was evaluated by PCR for genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc and by phenotypical tests for gelatinase, lipase, DNAse and α- and β-haemolysis. Most isolates (93·0%) harboured at least one lantibiotic or enterocin gene and were positive for several tested virulence genes, mainly asa1 (100%), gelE (93·0%) and efaA (83.7%). 53.5% of the isolates presented β-haemolysis [corrected]. Enterococcus spp. isolates presented an interesting potential application for food preservation because of bacteriocin production; however, virulence-related genes were identified in all RAPD profiles. The study demonstrated the contradictory characteristics of the tested Entero...Continue Reading

References

Jun 1, 1991·Applied and Environmental Microbiology·C B LewusT J Montville
Aug 26, 1998·International Journal of Food Microbiology·E C De Martinis, B D Franco
May 12, 2001·FEMS Microbiology Reviews·O McAuliffeC Hill
Mar 20, 2002·Research in Microbiology·Christine ArchimbaudBernard Joly
Jun 19, 2002·FEMS Microbiology Reviews·Giorgio Giraffa
Nov 9, 2002·Biochimie·Margaret A Riley, John E Wertz
May 16, 2003·Systematic and Applied Microbiology·Teresa SemedoRogério Tenreiro
Jun 18, 2003·International Journal of Food Microbiology·L De VuystH Revets
Nov 5, 2003·International Journal of Food Microbiology·Charles M A P FranzWilhelm H Holzapfel
Nov 5, 2003·International Journal of Food Microbiology·Giorgio Giraffa
Oct 8, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Güven Kayaoglu, Dag Ørstavik
May 5, 2005·International Journal of Antimicrobial Agents·Svetoslav D TodorovLeon M T Dicks
Jul 20, 2005·Journal of Basic Microbiology·Svetoslav D Todorov, Leon M T Dicks
Oct 12, 2005·International Journal of Food Microbiology·M R Foulquié MorenoL De Vuyst
Dec 3, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Patrice Courvalin
Feb 8, 2006·Applied and Environmental Microbiology·Ruth E WirawanJohn R Tagg
Oct 19, 2006·International Journal of Food Microbiology·Maria de Fátima Silva LopesMaria Teresa Barreto Crespo
Feb 15, 2007·FEMS Microbiology Reviews·Charles M A P FranzAntonio Gálvez
Sep 25, 2007·International Journal of Food Microbiology·Jean-Claude Ogier, Pascale Serror
Jun 11, 2008·Food Microbiology·Bruna C GomesElaine C P De Martinis
Apr 21, 2009·International Journal of Food Microbiology·Antonio M Martín-PlateroManuel Martínez-Bueno
May 19, 2009·International Journal of Food Microbiology·Svetoslav D Todorov, Leon M T Dicks
Sep 4, 2009·Trends in Microbiology·Antoni P A HendrickxWillem van Schaik
Sep 29, 2009·International Journal of Food Microbiology·Yasemin SterrHerbert Schmidt
Dec 4, 2009·Applied Biochemistry and Biotechnology·Manoj Kumar, Sheela Srivastava
Apr 20, 2010·International Journal of Food Microbiology·Haider KhanPak-Lam Yu
Apr 8, 2011·Current Pharmaceutical Biotechnology·Manuel Montalbán-LópezMercedes Maqueda
Oct 4, 2011·International Journal of Food Microbiology·Charles M A P FranzAntonio Gálvez

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Citations

Jun 25, 2014·International Journal of Food Microbiology·Luana Martins PerinLuís Augusto Nero
Aug 15, 2014·Probiotics and Antimicrobial Proteins·Ana Beatriz Jeronymo-CenevivaAna Lúcia B Penna
Aug 28, 2015·International Journal of Food Microbiology·Luana Martins PerinLuís Augusto Nero
Feb 19, 2015·Probiotics and Antimicrobial Proteins·Castro-Rodríguez DianaYáñez Fernández Jorge
Jun 4, 2016·Antonie van Leeuwenhoek·Luana Martins PerinLuís Augusto Nero
Jan 18, 2018·Journal of Food Science·Juliana Alves ResendeCláudio Galuppo Diniz
Apr 1, 2020·Animals : an Open Access Journal From MDPI·Renata F RabelloAloysio M F Cerqueira
Feb 25, 2017·Probiotics and Antimicrobial Proteins·Svetoslav Dimitrov TodorovLuís Augusto Nero
Jul 24, 2018·The Onderstepoort Journal of Veterinary Research·Shirwin PillayMatthew A Adeleke
Nov 18, 2018·The Journal of Dairy Research·Monique ColomboLuís Augusto Nero
Jan 26, 2020·Probiotics and Antimicrobial Proteins·Karina Maria Olbrich Dos SantosSvetoslav Dimitrov Todorov

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