Abstract
To provide molecular and phenotypical characterization of Enterococcus isolates obtained from raw milk and cheese, regarding their bacteriocinogenic and virulence activity. Forty-three bacteriocinogenic enterococci isolates were identified by 16s rDNA, fingerprinted by RAPD-PCR analysis and tested by PCR for the presence of genes for lantibiotics (lanM, lanB and lanC) and enterocins (entA, entB, entP, entL50AB and entAS48) and by phenotypical methods for bacteriocin production and inhibitory spectrum. Also, the virulence of the isolates was evaluated by PCR for genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc and by phenotypical tests for gelatinase, lipase, DNAse and α- and β-haemolysis. Most isolates (93·0%) harboured at least one lantibiotic or enterocin gene and were positive for several tested virulence genes, mainly asa1 (100%), gelE (93·0%) and efaA (83.7%). 53.5% of the isolates presented β-haemolysis [corrected]. Enterococcus spp. isolates presented an interesting potential application for food preservation because of bacteriocin production; however, virulence-related genes were identified in all RAPD profiles. The study demonstrated the contradictory characteristics of the tested Entero...Continue Reading
References
Jun 1, 1991·Applied and Environmental Microbiology·C B LewusT J Montville
Nov 1, 1993·Antimicrobial Agents and Chemotherapy·J W ChowM J Zervos
Apr 29, 1998·Journal of Bacteriology·L M CintasL S Håvarstein
Aug 26, 1998·International Journal of Food Microbiology·E C De Martinis, B D Franco
Apr 4, 2000·Journal of Applied Microbiology·M du ToitW H Holzapfel
Apr 3, 2001·Applied and Environmental Microbiology·T J Eaton, M J Gasson
May 12, 2001·FEMS Microbiology Reviews·O McAuliffeC Hill
Jan 10, 2002·Nature·Wolfgang HaasMichael S Gilmore
Mar 20, 2002·Research in Microbiology·Christine ArchimbaudBernard Joly
Jun 19, 2002·FEMS Microbiology Reviews·Giorgio Giraffa
Nov 9, 2002·Biochimie·Margaret A Riley, John E Wertz
May 16, 2003·Systematic and Applied Microbiology·Teresa SemedoRogério Tenreiro
Jun 18, 2003·International Journal of Food Microbiology·L De VuystH Revets
Nov 5, 2003·International Journal of Food Microbiology·Charles M A P FranzWilhelm H Holzapfel
Nov 5, 2003·International Journal of Food Microbiology·Giorgio Giraffa
Oct 8, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Güven Kayaoglu, Dag Ørstavik
Oct 9, 2004·Journal of Clinical Microbiology·Vanessa VankerckhovenHerman Goossens
May 5, 2005·International Journal of Antimicrobial Agents·Svetoslav D TodorovLeon M T Dicks
Jul 20, 2005·Journal of Basic Microbiology·Svetoslav D Todorov, Leon M T Dicks
Aug 16, 2005·Chest·Pablo RivasManuel L Fernández Guerrero
Aug 17, 2005·International Journal of Food Microbiology·M De KwaadstenietL M T Dicks
Oct 1, 2005·FEMS Microbiology Letters·Otto HyinkJohn R Tagg
Oct 12, 2005·International Journal of Food Microbiology·M R Foulquié MorenoL De Vuyst
Dec 3, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Patrice Courvalin
Feb 8, 2006·Applied and Environmental Microbiology·Ruth E WirawanJohn R Tagg
Jun 17, 2006·Antonie van Leeuwenhoek·Nivedita SharmaB Neopaney
Oct 19, 2006·International Journal of Food Microbiology·Maria de Fátima Silva LopesMaria Teresa Barreto Crespo
Feb 15, 2007·FEMS Microbiology Reviews·Charles M A P FranzAntonio Gálvez
Apr 6, 2007·Life Sciences·K S Girish, K Kemparaju
Sep 25, 2007·International Journal of Food Microbiology·Jean-Claude Ogier, Pascale Serror
Jun 11, 2008·Food Microbiology·Bruna C GomesElaine C P De Martinis
Apr 21, 2009·International Journal of Food Microbiology·Antonio M Martín-PlateroManuel Martínez-Bueno
May 19, 2009·International Journal of Food Microbiology·Svetoslav D Todorov, Leon M T Dicks
Sep 4, 2009·Trends in Microbiology·Antoni P A HendrickxWillem van Schaik
Sep 29, 2009·International Journal of Food Microbiology·Yasemin SterrHerbert Schmidt
Oct 21, 2009·Foodborne Pathogens and Disease·Maria Beatriz Tassinari OrtolaniLuís Augusto Nero
Dec 4, 2009·Applied Biochemistry and Biotechnology·Manoj Kumar, Sheela Srivastava
Apr 20, 2010·International Journal of Food Microbiology·Haider KhanPak-Lam Yu
Apr 27, 2010·Food Microbiology·Gabriela Nogueira ViçosaLuís Augusto Nero
Aug 19, 2010·Current Microbiology·Kaoutar BayoubAbdelaziz Soukri
Apr 8, 2011·Current Pharmaceutical Biotechnology·Manuel Montalbán-LópezMercedes Maqueda
Oct 4, 2011·International Journal of Food Microbiology·Charles M A P FranzAntonio Gálvez
Aug 1, 2007·Meat Science·Márcia A F BarrosVanerli Beloti
Citations
Dec 3, 2013·Food Microbiology·Lorenzo FavaroSvetoslav Dimitrov Todorov
Nov 8, 2013·Environmental Science & Technology·C VignaroliF Biavasco
Feb 14, 2014·BMC Microbiology·Luana Martins Perin, Luís Augusto Nero
Jun 25, 2014·International Journal of Food Microbiology·Luana Martins PerinLuís Augusto Nero
Aug 15, 2014·Probiotics and Antimicrobial Proteins·Ana Beatriz Jeronymo-CenevivaAna Lúcia B Penna
Jan 23, 2016·Frontiers in Microbiology·Annette C AndersonAli Al-Ahmad
Aug 28, 2015·International Journal of Food Microbiology·Luana Martins PerinLuís Augusto Nero
Mar 21, 2016·Journal of Dairy Science·Fen ZhangHua Wei
Mar 7, 2014·Environmental Microbiology Reports·Andrea Di CesareFrancesca Biavasco
Feb 19, 2015·Probiotics and Antimicrobial Proteins·Castro-Rodríguez DianaYáñez Fernández Jorge
Jun 4, 2016·Antonie van Leeuwenhoek·Luana Martins PerinLuís Augusto Nero
Dec 19, 2016·Journal of Dairy Science·Daniel M F AmaralAna Lúcia B Penna
Nov 24, 2017·Probiotics and Antimicrobial Proteins·Alaa Al SeraihDjamel Drider
Nov 12, 2013·Journal of Applied Microbiology·S C RibeiroC C G Silva
Jul 4, 2017·Frontiers in Microbiology·Lei WeiLianying Ma
Jan 18, 2018·Journal of Food Science·Juliana Alves ResendeCláudio Galuppo Diniz
Apr 1, 2020·Animals : an Open Access Journal From MDPI·Renata F RabelloAloysio M F Cerqueira
Feb 25, 2017·Probiotics and Antimicrobial Proteins·Svetoslav Dimitrov TodorovLuís Augusto Nero
Jun 15, 2019·Archives of Microbiology·Pierre Marie KaktchamMaría de Lourdes Pérez-Chabela
Feb 15, 2018·Frontiers in Microbiology·Nikola PopovićKatarina Veljović
Dec 31, 2016·Food Science and Biotechnology·Sine Özmen ToğayYonca Karagül Yüceer
Jul 24, 2018·The Onderstepoort Journal of Veterinary Research·Shirwin PillayMatthew A Adeleke
Nov 18, 2018·The Journal of Dairy Research·Monique ColomboLuís Augusto Nero
Feb 13, 2017·Journal of Dairy Science·V Q CavicchioliL A Nero
Jan 26, 2020·Probiotics and Antimicrobial Proteins·Karina Maria Olbrich Dos SantosSvetoslav Dimitrov Todorov
Jan 26, 2021·Frontiers in Veterinary Science·James Wabwire OguttuAgricola Odoi
Jul 13, 2021·Journal of Veterinary Research·Rui ZhongGuangneng Peng