Bacteriological and virulence study of a Mycobacterium chimaera isolate from a patient in China

Antonie van Leeuwenhoek
Guan LiuHai-Rong Huang

Abstract

A clinical isolate from a patient was identified as Mycobacterium chimaera, a recently identified species of nontuberculous Mycobacteria. The biochemical and molecular identity, drug sensitivity and virulence of this isolated strain were investigated. 16S rRNA, the 16S-23S ITS, hsp65 and rpoB were amplified, and their sequence similarities with other mycobacteria were analyzed. The minimum inhibitory concentrations of 22 anti-microbial agents against this isolate were established, and the virulence of the isolate was evaluated by intravenous injection into C57BL/6 mice using Mycobacterium tuberculosis H37Rv as a control strain. Growth and morphological characteristics and mycolic acid profile analysis revealed that this isolated strain was a member of the Mycobacterium avium complex. BLAST analysis of the amplified sequences showed that the isolated strain was closely related to M. chimaera. Susceptibility testing showed that the isolate was sensitive to rifabutin, rifapentine, clarithromycin, azithromycin, imipenem and cefoxitin. Bacterial load determination and tissue histopathology of the infected mice indicated that the isolate was highly virulent. The first case of M. chimaera infection in China was evaluated. The informat...Continue Reading

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Citations

Nov 5, 2016·Diagnostic Microbiology and Infectious Disease·Seong Mi MoonWon-Jung Koh
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Oct 30, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shannon H Kasperbauer, Charles L Daley
Oct 11, 2020·International Journal of Environmental Research and Public Health·Matteo BolcatoAnna Aprile

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