Bacteriophage Administration Reduces the Concentration of Listeria monocytogenes in the Gastrointestinal Tract and Its Translocation to Spleen and Liver in Experimentally Infected Mice.

International Journal of Microbiology
Volker MaiAlexander Sulakvelidze

Abstract

To investigate the efficacy of phage supplementation in reducing pathogen numbers, mice were treated via oral gavage with a Listeria monocytogenes phage preparation (designated ListShield) before being orally infected with L. monocytogenes. The concentrations of L. monocytogenes in the liver, spleen, and intestines were significantly lower (P < .05) in the phage-treated than in the control mice. Phage and antibiotic treatments were similarly effective in reducing the levels of L. monocytogenes in the internal organs of the infected mice. However, the significant weight loss detected in the control and antibiotic-treated groups was not observed in the infected, ListShield-treated mice. Long-term (90 days), biweekly treatment of uninfected mice with ListShield did not elicit detectable changes in the microbiota of their large intestines or deleterious changes in their health. Our data support the potential feasibility of using bacteriophages to control proliferation of L. monocytogenes in mice without affecting commensal microbiota composition.

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Citations

Feb 16, 2012·Bacteriophage·Stephen T AbedonElizabeth Martin Kutter
Nov 15, 2013·Bacteriophage·Alexandra Henein
Oct 16, 2016·Bacteriophage·Nitzan SofferAlexander Sulakvelidze
Oct 9, 2016·Folia Microbiologica·Sonika SharmaMohan G Vairale
Jan 13, 2015·Journal of Food Protection·Serena HeyseDuane Charbonneau
Oct 12, 2018·Applied Microbiology and Biotechnology·Hong-Duo BaoRan Wang
Jul 14, 2018·Genes·Hui Ling LeeMatthew Wook Chang
Sep 26, 2019·Frontiers in Microbiology·Upuli DissanayakeVolker Mai
Jan 17, 2017·Bacteriophage·A V AleshkinS S Bochkareva
Jun 18, 2021·Frontiers in Cellular and Infection Microbiology·Israa M Abd-AllahNadia A Hassouna
Aug 25, 2021·Critical Reviews in Microbiology·Paulina ŚliwkaAneta Skaradzińska

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Methods Mentioned

BETA
Electrophoresis

Software Mentioned

Quantity One
ListShield
Diversity Database
MS Exel

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