Bacteriophage-amplified bioluminescent sensing of Escherichia coli O157:H7

Analytical and Bioanalytical Chemistry
Steven RippGary Sayler

Abstract

Escherichia coli O157:H7 remains a continuous public health threat, appearing in meats, water, fruit juices, milk, cheese, and vegetables, where its ingestion at concentrations of perhaps as low as 10 to 100 organisms can result in potent toxin exposure and severe damage to the lining of the intestine. Abdominal pain and diarrhea develop, which in the very young or elderly can progress towards hemolytic uremic syndrome and kidney failure. To assist in the detection of E. coli O157:H7, a recombinant bacteriophage reporter was developed that uses quorum sensing (luxI/luxR) signaling and luxCDABE-based bioluminescent bioreporter sensing to specifically and autonomously respond to O157:H7 serotype E. coli. The bacteriophage reporter, derived from phage PP01, was tested in artificially contaminated foodstuffs including apple juice, tap water, ground beef, and spinach leaf rinsates. In apple juice, detection of E. coli O157:H7 at original inoculums of 1 CFU mL(-1) occurred within approximately 16 h after a 6-h pre-incubation, detection of 1 CFU mL(-1) in tap water occurred within approximately 6.5 h after a 6-h pre-incubation, and detection in spinach leaf rinsates using a real-time Xenogen IVIS imaging system resulted in detection o...Continue Reading

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Citations

Dec 18, 2010·Analytical and Bioanalytical Chemistry·Abby E Smartt, Steven Ripp
Nov 22, 2011·Analytical and Bioanalytical Chemistry·Abby E SmarttSteven Ripp
Jun 7, 2013·Analytical and Bioanalytical Chemistry·Elisa MicheliniAldo Roda
Mar 20, 2012·Applied and Environmental Microbiology·David A SchofieldIan J Molineux
Jan 13, 2010·Clinical Microbiology Reviews·Nicasio ManciniMassimo Clementi
Oct 16, 2009·Journal of Clinical Microbiology·David A SchofieldCaroline Westwater
Oct 17, 2015·Analytical and Bioanalytical Chemistry·Riikka PeltomaaMaría Cruz Moreno-Bondi
Apr 20, 2016·Frontiers in Microbiology·Jaewoo BaiJu-Hoon Lee
Sep 20, 2012·Bioengineered·David SchofieldIan J Molineux
Feb 18, 2014·Bacteriophage·Mathias Schmelcher, Martin J Loessner
Oct 11, 2012·Bacteriophage·David A SchofieldCaroline Westwater
Mar 25, 2014·The Analyst·R G van der MerweN C Gey van Pittius
Sep 24, 2016·Cell Chemical Biology·Ting-Yen Wei, Chao-Min Cheng
Jun 23, 2012·The Analyst·Amit SinghStephane Evoy
Mar 4, 2021·Nature Communications·Sierra M Brooks, Hal S Alper

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