Bacteriophage application restores ethanol fermentation characteristics disrupted by Lactobacillus fermentum

Biotechnology for Biofuels
Mei LiuElizabeth J Summer

Abstract

Contamination of corn mash by lactic acid bacteria (LAB) reduces the efficiency of the ethanol fermentation process. The industry relies heavily on antibiotics for contamination control and there is a need to develop alternative methods. The goals of this study were to determine the diversity and abundance of bacteria contaminating commercial ethanol fermentations, and to evaluate the potential of anti-LAB bacteriophages in controlling production losses. Bacterial populations in 27 corn mash samples collected from nine different commercial plants were determined by pyrosequencing of 16S rRNA amplicons. The results showed that the most abundant bacteria (>50 % of total population) in 24 of the 27 samples included LAB genera such as Lactobacillus, Streptococcus, Lactococcus, Weissella, Enterococcus, and Pediococcus. Lactobacillus was identified as the most prevalent genus at all fermentation stages in all plants, accounting for between 2.3 and 93.7 % of each population and constituting the major genus (>50 %) in nine samples from five plants and the most abundant genus in five other samples. Lactobacillus species, including L. delbrueckii, L. fermentum, L. mucosae, and L. reuteri were the most well-represented species. Two bacter...Continue Reading

References

Apr 29, 1997·International Journal of Food Microbiology·M E Stiles, W H Holzapfel
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 21, 1998·Nucleic Acids Research·A V Lukashin, M Borodovsky
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Jun 23, 2001·Journal of Industrial Microbiology & Biotechnology·N V NarendranathW M Ingledew
Sep 7, 2001·Annual Review of Microbiology·H Brussow
Jan 17, 2002·Cell·Harald Brüssow, Roger W Hendrix
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Sep 1, 2004·Journal of Industrial Microbiology & Biotechnology·Kelly A Skinner, Timothy D Leathers
Jun 28, 2005·Nucleic Acids Research·Peter SchattnerTodd M Lowe
Dec 15, 2005·Journal of Bacteriology·Elizabeth J SummerRy Young
Jun 6, 2006·Applied and Environmental Microbiology·Hélène DeveauSylvain Moineau
Oct 20, 2006·Archives of Virology·H-W Ackermann
Aug 30, 2007·Journal of Industrial Microbiology & Biotechnology·Kenneth M BischoffTimothy D Leathers
Jun 10, 2008·Applied and Environmental Microbiology·Simon J LabrieSylvain Moineau
Oct 23, 2008·Nucleic Acids Research·Sarah HunterCorin Yeats
Dec 17, 2008·Methods in Molecular Biology·Elizabeth J Summer
Dec 17, 2008·Methods in Molecular Biology·Sherwood R Casjens, Eddie B Gilcrease
Jan 17, 2009·Biotechnology and Bioengineering·Kenneth M BischoffJoseph O Rich
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Manuela Villion, Sylvain Moineau
Mar 17, 2009·Journal of Molecular Biology·Charles R StewartMarisa L Pedulla
Nov 10, 2009·Journal of Bacteriology·Elizabeth J SummerCarlos F Gonzalez
Dec 17, 2009·The Journal of Infectious Diseases·Lisa A CarmodyJohn J LiPuma
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Aug 18, 2010·Archives of Virology·Jochen KlumppHans-Wolfgang Ackermann
Nov 26, 2010·BMC Microbiology·Brigida T L LucenaMarcos Antonio de Morais
Nov 26, 2010·Applied and Environmental Microbiology·E J SummerR Young
Oct 26, 2011·Microbial Cell Factories·Josiane E Garneau, Sylvain Moineau
May 15, 2013·Applied and Environmental Microbiology·Jennifer MahonyDouwe van Sinderen
Sep 7, 2013·Frontiers in Microbiology·William J KellySinead C Leahy
Aug 29, 2014·Biotechnology for Biofuels·Juliano Bertozzi Silva, Dominic Sauvageau

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Citations

Sep 19, 2020·Biotechnology for Biofuels·Shao-Yeh LuChristopher D Skory
May 29, 2018·World Journal of Microbiology & Biotechnology·Sandra Regina Ceccato-Antonini
Jan 16, 2020·Current Microbiology·Timothy D LeathersMelinda S Nunnally
Nov 19, 2021·Environmental Science & Technology·Cory SchwarzPedro J J Alvarez

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Datasets Mentioned

BETA
KP027015
KP054477

Methods Mentioned

BETA
PCR
transmission electron microscopy

Software Mentioned

BLASTn
hmm
EcoInf
Artemis
InterProScan
BLASTp
Genemark

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