Bacteriophage-based nanoprobes for rapid bacteria separation

Nanoscale
Juhong ChenSam R Nugen

Abstract

The lack of practical methods for bacterial separation remains a hindrance for the low-cost and successful development of rapid detection methods from complex samples. Antibody-tagged magnetic particles are commonly used to pull analytes from a liquid sample. While this method is well-established, improvements in capture efficiencies would result in an increase of the overall detection assay performance. Bacteriophages represent a low-cost and more consistent biorecognition element as compared to antibodies. We have developed nanoscale bacteriophage-tagged magnetic probes, where T7 bacteriophages were bound to magnetic nanoparticles. The nanoprobe allowed the specific recognition and attachment to E. coli cells. The phage magnetic nanprobes were directly compared to antibody-conjugated magnetic nanoprobes. The capture efficiencies of bacteriophages and antibodies on nanoparticles for the separation of E. coli K12 at varying concentrations were determined. The results indicated a similar bacteria capture efficiency between the two nanoprobes.

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Citations

Sep 25, 2015·ACS Applied Materials & Interfaces·Juhong ChenSam R Nugen
Aug 16, 2016·Biosensors & Bioelectronics·Kun WangShaojun Dong
Jun 21, 2016·Journal of Applied Microbiology·P BárdyJ Doškař
Dec 17, 2016·Analytical and Bioanalytical Chemistry·Meng-Qi HeRong-Huan He
Dec 20, 2016·Bioconjugate Chemistry·Marta JanczukRobert Hołyst
Dec 15, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Kathleen DavisO Thompson Mefford
Dec 13, 2016·Chemical Society Reviews·Juhong ChenVincent M Rotello
Jul 6, 2019·Frontiers in Bioengineering and Biotechnology·Chen XuAiguo Wu
Nov 12, 2020·Small·Nanjing HaoTony Jun Huang
Mar 5, 2021·Bioconjugate Chemistry·Caitlin M CarmodySam R Nugen

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