PMID: 6975715Oct 1, 1981Paper

Bacteriophage fd gene-2 protein. Processing of phage fd viral strands replicated by phage T7 enzymes

European Journal of Biochemistry
G HarthK Geider

Abstract

Bacteriophage T7 gene 4 protein and DNA polymerase of the phage were used to study the viral strand synthesis of bacteriophage fd in vitro. Cleavage of supercoiled phage fd replicative form (RF) by fd gene 2 protein produced a nick at a specific site in the viral strand. The cleaved double-stranded DNA was unwound by T7 gene 4 protein and T7 DNA polymerase and the 3' end of the nicked strand simultaneously extended according to the rolling circle mechanism. After a complete round of DNA synthesis fd gene 2 protein cleaved the viral strand presumably at the same site, where the endonuclease cuts fd RF I, and subsequently sealed the single-stranded linear DNA into a circle. The reaction products were analyzed by velocity sedimentation, gel electrophoresis and electron microscopy. Most of the single-stranded DNA synthesized was circular. No host proteins were required for the formation of the single-stranded circles. Strand switching of the T7 DNA polymerase indicated by double-stranded tails of the rolling circle structures reduced the yield of viral single-stranded circles in this enzyme system.

References

Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·C C RichardsonD S Richardson
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·K Geider, T F Meyer
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S EisenbergA Kornberg
Feb 1, 1977·European Journal of Biochemistry·E ScherzingerA Yuki
Dec 14, 1972·Journal of Molecular Biology·N S Lin, D Pratt
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D BrutlagA Kornberg

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Citations

Aug 1, 1990·Idengaku zasshi·K Horiuchi
Apr 29, 1982·Nature·T F Meyer, K Geider
Jun 24, 1985·Biochimica Et Biophysica Acta·P D Baas
Jun 1, 1985·Microbiological Reviews·N D Zinder, K Horiuchi
Dec 1, 1986·Microbiological Reviews·I Rasched, E Oberer
Sep 5, 1988·Journal of Molecular Biology·W Fulford, P Model

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