Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions

Frontiers in Microbiology
Giantommaso ScarasciaPei-Ying Hong

Abstract

Pseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P. aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacteriophages showed optimal infectivity at a multiplicity of infection of 10 in saline conditions, and demonstrated lytic abilities over all tested temperature (25, 30, 37, and 45°C) and pH 6-9. Planktonic P. aeruginosa exhibited significantly longer lag phase and lower specific growth rates upon exposure to bacteriophages. Bacteriophages were subsequently applied to P. aeruginosa-enriched biofilm and were determined to lower the relative abundance of Pseudomonas-related taxa from 0.17 to 5.58% in controls to 0.01-0.61% in treated microbial communities. The relative abundance of Alphaproteobacteria, Pseudoalteromonas, and Planococcaceae decreased, possibly due to the phage-induced disruption of the biofilm matrix. Lastly, when applied to mitigate biofouling of ultrafiltration membranes, bacteriophages were determined to reduce the transmembrane pressure increase by 18% when utilized alone, and by 49% when used in combination with citric ac...Continue Reading

References

Jan 1, 1972·Annual Review of Genetics·H Echols
Jun 4, 2004·Journal of Bacteriology·Sandra Chibani-ChennoufiHarald Brüssow
Aug 30, 2005·Trends in Microbiology·Vincent A Fischetti
Mar 28, 2007·Antimicrobial Agents and Chemotherapy·Catherine S McVayJoe A Fralick
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Oct 24, 2007·Journal of Applied Microbiology·J LangletC Gantzer
Apr 18, 2009·Water Research·Lauren F GreenleePhilippe Moulin
Mar 30, 2010·Nature Reviews. Microbiology·Simon J LabrieSylvain Moineau
Apr 7, 2010·Applied and Environmental Microbiology·Alexandra DheillyAlain Dufour
Jun 1, 2011·Folia Microbiologica·E JończykA Górski
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Aug 14, 2012·Biotechnology and Bioengineering·Yanyan Zhang, Zhiqiang Hu
Feb 26, 2013·Applied and Environmental Microbiology·Zeinab HosseinidoustTheo G M van de Ven
Jul 31, 2013·Applied and Environmental Microbiology·Zeinab HosseinidoustNathalie Tufenkji
Oct 12, 2013·Current Opinion in Microbiology·Ry Young
Mar 4, 2014·The Journal of Microbiology·Ryland Young
Mar 7, 2014·Frontiers in Microbiology·Mai Huong Ly-Chatain
Nov 25, 2014·Biophysical Journal·Rikkert J NapRudolf Podgornik
Mar 3, 2015·Biotechnology and Bioengineering·Ananda Shankar BhattacharjeeRamesh Goel
Apr 29, 2015·Water Research·Muhammad Tariq KhanJean Philippe Croue
May 15, 2015·Journal of Virology·Diana P PiresJoana Azeredo

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Citations

Sep 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Giantommaso ScarasciaPei-Ying Hong

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Datasets Mentioned

BETA
PRJEB23782

Methods Mentioned

BETA
membrane filtration
amplicon sequencing
transmission electron microscopy
electrophoresis
flow cytometry

Software Mentioned

UCHIME
BLASTN
Primer
SIMPER

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