PMID: 107392Feb 26, 1979

Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site

Molecular & General Genetics : MGG
C PourcelP Tiollais

Abstract

Bacteriophage lambda vectors, derived from lambda plac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the beta-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac- and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter. Mutations Qam73 and Sam7 greatly increase the amount of beta-galactosidase synthesized by the vector bacteriophage. The lambda ZEQS vector has been certified B2 (EK2) by the French control commission "Recombinaisons génétiques in vitro".

References

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Citations

Sep 1, 1992·Biotechnology and Bioengineering·N PadukoneD F Ollis
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P CharnayP Tiollais
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C MarchalM Hofnung
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A DejeanS Wain-Hobson
Oct 22, 2013·Nucleic Acids Research·Wil A M LoenenN E Murray
Jan 1, 1982·Journal of Cellular Biochemistry·A DejeanS Wain-Hobson

Related Concepts

Lactrase
Coliphages
Genes, Spliced
DNA, Viral
SDS-PAGE
Alkalescens-Dispar Group
Galactosidase
Genes, Viral
Lac Gene

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