Bacteriophage lambda gpNu1 and Escherichia coli IHF proteins cooperatively bind and bend viral DNA: implications for the assembly of a genome-packaging motor

Biochemistry
M Ortega, Carlos Enrique Catalano

Abstract

Terminase enzymes are common to both prokaryotic and eukaryotic double-stranded DNA viruses and are responsible for packaging viral DNA into the confines of an empty procapsid shell. In all known cases, the holoenzymes are heteroligomers composed of a large subunit that possesses the catalytic activities required for genome packaging and a small subunit that is responsible for specific recognition of viral DNA. In bacteriophage lambda, the DNA recognition protein is gpNu1. The gpNu1 subunit interacts with multiple recognition elements within cos, the packaging initiation site in viral DNA, to site-specifically assemble the packaging machinery. Motor assembly is modulated by the Escherichia coli integration host factor protein (IHF), which binds to a consensus sequence also located within cos. On the basis of a variety of biochemical data and the recently solved NMR structure of the DNA binding domain of gpNu1, we proposed a novel DNA binding mode that predicts significant bending of duplex DNA by gpNu1 (de Beer et al. (2002) Mol. Cell 9, 981-991). We further proposed that gpNu1 and IHF cooperatively bind and bend viral DNA to regulate the assembly of the packaging motor. Here, we characterize cooperative gpNu1 and IHF binding t...Continue Reading

References

Jan 1, 1991·Annual Review of Biochemistry·H Murialdo
Sep 8, 1995·Journal of Molecular Biology·R R Higgins, A Becker
Jan 1, 1995·Methods in Enzymology·E Di CeraA Vindigni
Feb 6, 2002·Journal of Molecular Biology·Gauri M DhavanMichael Brenowitz
Mar 10, 2005·Journal of Molecular Biology·Nasib K MalufCarlos Enrique Catalano

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Citations

Dec 24, 2011·Biochemistry·Jenny R ChangCarlos E Catalano
Dec 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Siyang SunVenigalla B Rao
Dec 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Carina R BüttnerAlfred A Antson
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Benjamin T Andrews, Carlos Enrique Catalano
Nov 27, 2010·Nucleic Acids Research·Manjira Ghosh-KumarVenigalla B Rao
Jan 5, 2007·Journal of Virology·Nelli J KarhuJaana K H Bamford
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sergei KhrapunovCarlos Enrique Catalano
Sep 9, 2008·Journal of Molecular Biology·Daniel NemecekGeorge J Thomas
May 10, 2015·Virology·Michael FeissJean Sippy
Oct 22, 2015·Biophysical Journal·Teng-Chieh YangCarlos Enrique Catalano
Jul 8, 2014·Virology·Venkata ChelikaniKiran Kondabagil
Oct 20, 2007·Journal of Molecular Biology·Daniel NemecekGeorge J Thomas
May 6, 2016·PloS One·Choon Seok OhMichael Feiss
May 16, 2007·Molecular Microbiology·Peixuan Guo, Tae Jin Lee
Jun 15, 2016·Physical Review. E·Tanmoy Pal, Somendra M Bhattacharjee
Apr 27, 2017·Biophysical Journal·Teng-Chieh YangCarlos E Catalano

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