Bacteriophage-mediated Glucosylation Can Modify Lipopolysaccharide O-Antigens Synthesized by an ATP-binding Cassette (ABC) Transporter-dependent Assembly Mechanism.

The Journal of Biological Chemistry
Evan MannC Whitfield

Abstract

Lysogenic bacteriophages may encode enzymes that modify the structures of lipopolysaccharide O-antigen glycans, altering the structure of the bacteriophage receptor and resulting in serotype conversion. This can enhance virulence and has implications for antigenic diversity and vaccine development. Side chain glucosylation is a common modification strategy found in a number of bacterial species. To date, glucosylation has only been observed in O-antigens synthesized by Wzy-dependent pathways, one of the two most prevalent O-antigen synthesis systems. Here we exploited a heterologous system to study the glucosylation potential of a model O-antigen produced in an ATP-binding cassette (ABC) transporter-dependent system. Although O-antigen production is cryptic in Escherichia coli K-12, because of a mutation in the synthesis genes, it possesses a prophage glucosylation cluster, which modifies the GlcNAc residue in an α-l-Rha-(1→3)-d-GlcNAc motif found in the original O16 antigen. Raoultella terrigena ATCC 33257 produces an O-antigen possessing the same disaccharide motif, but its assembly uses an ABC transporter-dependent system. E. coli harboring the R. terrigena O-antigen biosynthesis genes produced an O-antigen displaying reduce...Continue Reading

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Citations

Feb 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Danielle M WilliamsChris Whitfield
Sep 1, 2017·Immunological Reviews·Peter DeinesThomas C G Bosch
Feb 13, 2020·Biotechnology & Genetic Engineering Reviews·Thomas E MacCalmanStephen E Harding
Mar 15, 2020·The Journal of Biological Chemistry·Ilias TheodorouDouwe van Sinderen
May 20, 2020·The Journal of Biological Chemistry·Chris WhitfieldSteven D Kelly
Aug 17, 2018·Viruses·Sonja KunstmannStefanie Barbirz
Apr 1, 2018·The Journal of Biological Chemistry·Bradley R ClarkeChris Whitfield
Jan 30, 2021·The Journal of Biological Chemistry·Ilias TheodorouDouwe van Sinderen
May 13, 2021·Journal of the American Chemical Society·Magdalena Jancheva, Thomas Böttcher
Nov 3, 2021·ACS Chemical Biology·Harald NothaftChristine M Szymanski

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