Bacteriophages of wastewater foaming-associated filamentous Gordonia reduce host levels in raw activated sludge

Scientific Reports
Mei LiuElizabeth J Summer

Abstract

Filamentous bacteria are a normal and necessary component of the activated sludge wastewater treatment process, but the overgrowth of filamentous bacteria results in foaming and bulking associated disruptions. Bacteriophages, or phages, were investigated for their potential to reduce the titer of foaming bacteria in a mixed-microbial activated sludge matrix. Foaming-associated filamentous bacteria were isolated from activated sludge of a commercial wastewater treatment plan and identified as Gordonia species by 16S rDNA sequencing. Four representative phages were isolated that target G. malaquae and two un-named Gordonia species isolates. Electron microscopy revealed the phages to be siphophages with long tails. Three of the phages--GordTnk2, Gmala1, and GordDuk1--had very similar ~76 kb genomes, with >93% DNA identity. These genomes shared limited synteny with Rhodococcus equi phage ReqiDocB7 and Gordonia phage GTE7. In contrast, the genome of phage Gsput1 was smaller (43 kb) and was not similar enough to any known phage to be placed within an established phage type. Application of these four phages at MOIs of 5-15 significantly reduced Gordonia host levels in a wastewater sludge model by approximately 10-fold as compared to n...Continue Reading

References

Mar 1, 1980·Applied and Environmental Microbiology·D L Ewert, M J Paynter
Aug 1, 1996·The Australian and New Zealand Journal of Surgery·P H ChapuisI G Cunningham
Mar 21, 1998·Nucleic Acids Research·A V Lukashin, M Borodovsky
Mar 9, 1999·Antonie van Leeuwenhoek·M GoodfellowJ Chun
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Feb 22, 2001·Antimicrobial Agents and Chemotherapy·A SulakvelidzeJ G Morris
Sep 7, 2001·Annual Review of Microbiology·W C Summers
Aug 22, 2002·Water Research·Munawwar Ali KhanTakashi Mino
Nov 27, 2002·Antonie van Leeuwenhoek·Michael WagnerHolger Daims
May 24, 2003·Applied Microbiology and Biotechnology·M A SékaW Verstraete
May 31, 2003·Nature Reviews. Drug Discovery·Carl R MerrilSankar L Adhya
Feb 11, 2004·Water Research·António M P MartinsMark C M van Loosdrecht
Mar 3, 2005·The Science of the Total Environment·S WitheyT Stephenson
Nov 1, 2005·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Shigenobu MatsuzakiShosuke Imai
May 1, 2007·FEMS Microbiology Ecology·Caroline KragelundPer Halkjaer Nielsen
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Oct 23, 2008·Nucleic Acids Research·Sarah HunterCorin Yeats
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Dec 17, 2008·Methods in Molecular Biology·Elizabeth J Summer
Feb 17, 2009·Applied and Environmental Microbiology·Lachlan SpeirsRobert J Seviour
Jul 23, 2009·FEMS Microbiology Reviews·Per Halkjaer NielsenJeppe Lund Nielsen
Oct 29, 2009·BMC Bioinformatics·Mira V Han, Christian M Zmasek
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Jun 10, 2010·Annual Review of Microbiology·Graham F Hatfull
Nov 26, 2010·Applied and Environmental Microbiology·E J SummerR Young
Dec 25, 2010·Applied and Environmental Microbiology·Steve PetrovskiDaniel Tillett
Jan 18, 2011·Water Research·Steve PetrovskiRobert J Seviour
Aug 11, 2011·Bioengineered Bugs·Jeongdong ChoiRamesh Goel
Sep 20, 2011·Applied and Environmental Microbiology·Steve PetrovskiDaniel Tillett
Jan 28, 2012·Journal of Virology·Graham F HatfullUNKNOWN Phage Hunters Integrating Research and Education Program
Aug 21, 2014·Biotechnology Research International·Krishna KhairnarWaman N Paunikar

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Citations

Mar 1, 2016·Frontiers in Microbiology·Nataliya M ShchegolkovaAnna V Kudryavtseva
Jun 25, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Jul 2, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Jul 2, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Aug 16, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Aug 16, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Aug 16, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Aug 16, 2016·Genome Announcements·Matthew T MontgomeryGraham F Hatfull
Aug 20, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Jul 9, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Jul 2, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Apr 6, 2017·Environmental Science and Pollution Research International·Agnieszka Pajdak-StósEdyta Fiałkowska
Jul 25, 2019·Pathogens·Steven BatinovicAshley E Franks
Nov 24, 2020·Bioinformatics·Travis N MavrichGraham F Hatfull
Dec 10, 2020·Frontiers of Environmental Science & Engineering·Mengzhi JiQiang Li
Mar 29, 2021·BMC Microbiology·Siew Herng ChanDiane McDougald
Jun 29, 2021·Applied Microbiology and Biotechnology·Ruyin LiuXinchun Liu
Aug 28, 2021·International Journal of Environmental Research and Public Health·Paula RogovskiGislaine Fongaro
Nov 19, 2021·Environmental Science & Technology·Cory SchwarzPedro J J Alvarez

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Datasets Mentioned

BETA
GTE7
KR067658
KR067659
KR067660
KR067661
KR067662
KR067663
KR067664
KP790008
KP790009

Methods Mentioned

BETA
phage treatment
PCR

Software Mentioned

InterProScan
Genemark
ClustalX
Artemis
hmm
BLASTp
Archaeopteryx
RDP SeqMatch

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