Bacteriorhodopsin/amphipol complexes: structural and functional properties

Biophysical Journal
Yann GohonChristine Ebel

Abstract

The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic flash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that in its native membrane. BR/APol complexes form well defined, globular particles comprising a monomer of BR, a complete set of purple membrane lipids, and, in a peripheral distribution, approximately 2 g APol/g BR, arranged in a compact layer. In the absence of free APol, BR/APol particles can autoassociate into small or large ordered fibrils.

References

May 1, 1981·Quarterly Reviews of Biophysics·H Eisenberg
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·C TribetJ L Popot
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·D I SvergunG Zaccai
Mar 22, 2000·Biochemistry·A CorcelliM Kates
Apr 5, 2000·The Journal of Biological Chemistry·P ChampeilM le Maire
Sep 14, 2000·Biochimica Et Biophysica Acta·G Váró
Aug 9, 2002·Nature·Chikashi Toyoshima, Hiromi Nomura
Nov 1, 2002·Biochimica Et Biophysica Acta·Richard NeutzeEhud M Landau
Jun 27, 2003·Nature·Atsuo MiyazawaNigel Unwin
Aug 19, 2003·Journal of Lipid Research·Simona LobassoAngela Corcelli
Sep 30, 2003·Cellular and Molecular Life Sciences : CMLS·J-L PopotC Tribet
Mar 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·G J Kleywegt, T A Jones
Oct 21, 2004·Analytical Biochemistry·Yann GohonChristine Ebel
Jun 3, 2005·Journal of Lipid Research·Christian RennerDieter Oesterhelt
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Manuela ZoonensJean-Luc Popot
Jan 25, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Yann GohonJean-Luc Popot
Feb 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yajing LiKevin Redding
Nov 23, 2006·Biochemistry·Cosmin L PocanschiJean-Luc Popot
Sep 11, 2007·Biochimica Et Biophysica Acta·C DiabF M Winnik

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Citations

Aug 29, 2012·European Biophysics Journal : EBJ·Tassadite DahmaneJean-Luc Popot
Aug 3, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Jason D PerlmutterJonathan N Sachs
Jul 3, 2013·Nature Methods·Houchao TaoQinghai Zhang
Jan 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Delphine CharvolinJean-Luc Popot
Sep 13, 2011·The Journal of Biological Chemistry·Teresa L CvetkovVera Y Moiseenkova-Bell
Jul 16, 2013·The European Physical Journal. E, Soft Matter·Cécile BreytonChristine Ebel
May 7, 2011·Annual Review of Biophysics·J-L PopotM Zoonens
Jul 30, 2009·European Biophysics Journal : EBJ·Laurent J CatoireJean-Luc Popot
Dec 10, 2015·Biochemistry·Jean-Luc Popot, Donald M Engelman
Apr 19, 2011·Trends in Biotechnology·Jean-Louis BanèresBernard Mouillac
Jun 24, 2010·Current Opinion in Structural Biology·Thomas RaschleGerhard Wagner
Oct 23, 2015·Biomacromolecules·Fabrice GiustiManuela Zoonens
Aug 5, 2014·The Journal of Membrane Biology·Yann FerrandezPhilippe Minard
Aug 12, 2014·The Journal of Membrane Biology·Milena OpačićJean-Luc Popot
Mar 4, 2014·The Journal of Membrane Biology·Wanatchaporn ArunmaneeJeremy H Lakey
Mar 22, 2014·The Journal of Membrane Biology·Fabrice GiustiJean-Luc Popot
Apr 4, 2014·The Journal of Membrane Biology·Christel Le BonFabrice Giusti
Apr 15, 2014·The Journal of Membrane Biology·Eduardo Antonio Della PiaKaren L Martinez
Sep 7, 2014·The Journal of Membrane Biology·V PolovinkinV Gordeliy
Sep 7, 2014·The Journal of Membrane Biology·V PolovinkinV Gordeliy
Sep 11, 2014·The Journal of Membrane Biology·Moeava TeheiJean-Luc Popot
May 27, 2014·The Journal of Membrane Biology·Shantha ElterGerhard Wagner
May 28, 2014·The Journal of Membrane Biology·Aleksandr SverzhinskyJames W Coulton
Jun 5, 2014·The Journal of Membrane Biology·Kevin W HuynhVera Y Moiseenkova-Bell
Jun 20, 2014·The Journal of Membrane Biology·Delphine CharvolinJean-Luc Popot
Jun 16, 2014·The Journal of Membrane Biology·Jason D PerlmutterJonathan N Sachs
Jun 28, 2014·The Journal of Membrane Biology·Manuela Zoonens, Jean-Luc Popot
Sep 13, 2011·The EMBO Journal·Thorsten AlthoffWerner Kühlbrandt
Oct 28, 2014·PloS One·Michael StanglDirk Schneider
Jul 22, 2010·Nanoscale·Yi-Ping Ho, Kam W Leong
Jan 31, 2014·ACS Nano·Eduardo Antonio Della PiaKaren Laurence Martinez
Jan 4, 2019·Acta Crystallographica. Section D, Structural Biology·Wanatchaporn ArunmaneeJeremy H Lakey
Jul 21, 2016·Nature·Eamon F X ByrneChristian Siebold
Aug 13, 2014·The Journal of Biological Chemistry·Melina A AgostoTheodore G Wensel
Jun 24, 2016·Beilstein Journal of Nanotechnology·Saurav KumarAmol P Bhondekar

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