Bacterium-based heavy metal biosorbents: enhanced uptake of cadmium by E. coli expressing a metallothionein fused to beta-galactosidase

BioTechniques
N YoshidaY Murooka

Abstract

We investigated the potential utility of a recombinant E. coli that expresses the human metallothionein II gene as a fusion protein with beta-galactosidase as a heavy metal biosorbent. E. coli cells expressing the metallothionein fusion demonstrated enhanced binding of Cd2+ compared to cells that lack the metallothionein. It was shown that the metallothionein fusion was capable of efficiently removing Cd2+ from solutions. Approximately 40% of the Cd2+ accumulated by the recombinant cells free in suspension was associated with the outer cell membrane, and 60% of that was present in the cytoplasm.

Citations

Aug 9, 2005·Applied and Environmental Microbiology·Naghma NazGeoffrey M Gadd
Mar 7, 2003·Applied and Environmental Microbiology·Rutchadaporn SriprangYoshikatsu Murooka
Nov 14, 2018·Frontiers in Bioengineering and Biotechnology·Patrick DiepAlexander F Yakunin

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