Bacteroides fragilis Strain ZY-312 Defense against Cronobacter sakazakii-Induced Necrotizing Enterocolitis In Vitro and in a Neonatal Rat Model

MSystems
Hong ying FanFachao Zhi

Abstract

Cronobacter sakazakii is an important pathogen associated with the development of necrotizing enterocolitis (NEC), infant sepsis, and meningitis. Several randomized prospective clinical trials demonstrated that oral probiotics could decrease the incidence of NEC. Previously, we isolated and characterized a novel probiotic, Bacteroides fragilis strain ZY-312. However, it remains unclear how ZY-312 protects the host from the effects of C. sakazakii infection. To understand the underlying mechanisms triggering the probiotic effects, we tested the hypothesis that there was cross talk between probiotics/probiotics-modulated microbiota and the local immune system, governed by the permeability of the intestinal mucosa, using in vitro and in vivo models for the intestinal permeability. The probiotic effects of ZY-312 on intestinal epithelial cells were first examined, and the results revealed that ZY-312 inhibited C. sakazakii invasion, C. sakazakii-induced dual cell death (pyroptosis and apoptosis), and epithelial barrier dysfunction in vitro and in vivo The presence of ZY-312 also resulted in decreased expression of an inflammasome (NOD-like receptor family member pyrin domain-containing protein 3 [NLRP3]), caspase-3, and serine prot...Continue Reading

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Citations

Aug 8, 2020·Critical Reviews in Food Science and Nutrition·Chen WangWei Chen
Feb 14, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ling ZongXin Wang
Dec 11, 2021·Journal of the American Chemical Society·Gang FanAriel L Furst

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Datasets Mentioned

BETA
PRJNA547866

Methods Mentioned

BETA
fluorescence microscopy
enzyme-linked immunosorbent assay
Illumina sequencing
flow cytometry
protein assay

Software Mentioned

UPARSE
SPSS
FLASH

Related Concepts

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