Bacteroides fragilis toxin rapidly intoxicates human intestinal epithelial cells (HT29/C1) in vitro.

Infection and Immunity
R F Saidi, C L Sears

Abstract

Enterotoxigenic Bacteroides fragilis strains associated with childhood diarrhea produce a 20-kDa protein toxin (BFT). Purified BFT causes striking morphologic changes in subconfluent human colonic epithelial cells (HT29/C1). In a 3-h HT29/C1 cell assay, the estimated half-maximal effective concentration of BFT was 12.5 pM, and morphologic effects were detectable as early as 30 min and nearly complete by 1.5 h. Concentrations as low as 0.5 pM could also cause intoxication, but morphologic changes were detectable only when the assay was extended to 18 h. The onset of this intoxication was concentration dependent and rapid, occurring within minutes (<7 min at 0.25 nM, <2 min at 2.5 nM). Notably, the onset of intoxication at 37 degrees C became irreversible to washing within 2 min after exposure to BFT. Morphologic changes were completely inhibited by treatment of HT29/C1 cells with BFT at 4 degrees C but could be demonstrated by subsequent warming to temperatures of 15 degrees C or higher after washing. The time required for the association of BFT with HT29/C1 cells at 4 degrees C was inversely correlated with concentration. Inhibitors of endosomal and Golgi trafficking (NH4Cl and brefeldin A) prevented the intoxication of HT29/C1...Continue Reading

References

May 1, 1977·Annals of Internal Medicine·B F Polk, D L Kasper
Apr 1, 1992·Infection and Immunity·R L Van TassellT D Wilkins
Aug 1, 1991·The Journal of Infectious Diseases·T A GomesM R Toledo
Jan 1, 1990·Reviews of Infectious Diseases·R L GuerrantJ Crane
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D TranL Orci
Jul 21, 1988·Biochimica Et Biophysica Acta·I R MellorM S Sansom
Aug 1, 1988·The Journal of Clinical Investigation·P A HuottK Dharmsathaphorn
Jun 1, 1987·Microbial Pathogenesis·B HenriquesM Thelestam
May 1, 1987·Journal of Medical Microbiology·T J MitchellJ Stephen
May 1, 1974·Applied Microbiology·W E Moore, L V Holdeman
Jun 1, 1969·The Journal of Infectious Diseases·W E MooreL V Holdeman
May 31, 1984·Biochemical and Biophysical Research Communications·G T Keusch, M Jacewicz
May 1, 1984·Infection and Immunity·L L MyersM M Border
Dec 19, 1983·Biochimica Et Biophysica Acta·I Florin, M Thelestam
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·N KatoK Ueno
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C L SearsR L Van Tassell
Jun 9, 1995·The Journal of Biological Chemistry·I JustK Aktories
Jan 1, 1995·Infection and Immunity·J S MoncriefT D Wilkins
Dec 1, 1994·The Journal of Cell Biology·R G PartonK Simons
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M TrucksisJ B Kaper
Aug 1, 1993·Infection and Immunity·S T DontaT K Tomicic
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L A DreyfusS J Morris
Sep 1, 1995·Protein Science : a Publication of the Protein Society·M SukumarL M Gierasch
Jan 1, 1995·Scandinavian Journal of Infectious Diseases·V H San JoaquinC L Sears
Sep 1, 1994·Clinical and Diagnostic Laboratory Immunology·R L Van TassellT D Wilkins
Aug 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L M Mundy, C L Sears

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Citations

Apr 16, 2002·Infection and Immunity·Shaoguang WuCynthia L Sears
Apr 16, 2009·Clinical Microbiology Reviews·Cynthia L Sears
Jun 21, 2014·BioMed Research International·Rita C RuizRoxane M F Piazza
Aug 24, 2006·Infection and Immunity·Shaoguang WuCynthia L Sears
Aug 6, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cynthia L SearsFirdausi Qadri
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·S WuC L Sears
Aug 12, 2014·The Journal of Clinical Investigation·Cynthia L SearsFranck Housseau
Jun 24, 2016·The Open Microbiology Journal·Payam Fathi, Shaoguang Wu
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·C L Sears
Mar 1, 1997·Infection and Immunity·A A FrancoC L Sears
Nov 9, 2021·Clinical & Experimental Metastasis·Charlotte HenstraAnika Nagelkerke

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