Baculovirus Actin-Based Motility Drives Nuclear Envelope Disruption and Nuclear Egress

Current Biology : CB
T Ohkawa, Matthew D Welch

Abstract

Viruses that replicate in the host cell nucleus face challenges in usurping cellular pathways to enable passage through the nuclear envelope [1]. Baculoviruses are enveloped, double-stranded DNA viruses that infect lepidopteran insects and are tools for protein expression, cell transduction, and pest management [2-4]. The type species Autographa californica M nucleopolyhedrovirus (AcMNPV) shares with other pathogens an ability to assemble host actin monomers (G-actin) into actin filaments (F-actin) to drive motility [5]. During early infection, actin-based motility in the cytoplasm speeds AcMNPV transit to the nucleus and passage through nuclear pores, enabling nuclear ingress [6, 7]. During late infection, AcMNPV assembles F-actin within the nucleus [8], which is essential for virus production [9, 10]. However, the function of nuclear F-actin is poorly understood [11], and its mechanistic role in AcMNPV infection was unknown. We show that AcMNPV mobilizes actin within the nucleus to promote egress. AcMNPV nucleocapsids exhibit intranuclear actin-based motility, mediated by the viral protein P78/83 and the host Arp2/3 complex. Viral motility drives transit to the nuclear periphery and is required for viruses to enter protrusion...Continue Reading

Citations

Sep 13, 2019·Journal of Virology·Tongtong WangYong Huang
Sep 5, 2019·Nature Cell Biology·Christopher Patrick CaridiIrene Chiolo
May 3, 2020·Neotropical Entomology·D R Sosa-GómezB M Ribeiro
May 21, 2020·Science China. Life Sciences·Xia LiXian-En Zhang
Dec 9, 2020·Cold Spring Harbor Perspectives in Biology·Svenja UlfertsMaria K Vartiainen
Apr 4, 2021·International Journal of Molecular Sciences·Pramod PanthaMaheshi Dassanayake
Aug 29, 2021·Viruses·Ayda KhorramnejadLaila Gasmi
Oct 29, 2021·Molecular Systems Biology·Xiaonan LiuMarkku Varjosalo

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