Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery

Briefings in Functional Genomics & Proteomics
Christian Oker-BlomR Grabherr

Abstract

Recombinant baculoviruses have been extensively used as vectors for abundant expression of a large variety of foreign proteins in insect cell cultures. The appeal of the system lies essentially in easy cloning techniques and virus propagation combined with the eukaryotic post-translational modification machinery of the insect cell. Recently, a novel molecular biology tool was established by the development of baculovirus surface display, using different strategies for presentation of foreign peptides and proteins on the surface of budded virions. This eukaryotic display system enables presentation of large complex proteins on the surface of baculovirus particles and has thereby become a versatile system in molecular biology. Surface display strategies play an important role, as they may be used to enhance the efficiency and specificity of viral binding and entry to mammalian cells. In addition, baculovirus surface display vectors have been engineered to contain mammalian promoter elements designed for gene delivery both in vitro and in vivo. Moreover, baculovirus capsid display has recently been developed; this holds promise for intracellular targeting of the viral capsid and subsequent cytosolic delivery of desired protein moi...Continue Reading

Citations

May 7, 2005·Nature Biotechnology·Thomas A KostDonald L Jarvis
Jan 10, 2013·Lab on a Chip·Yun ShuZhi-Ling Zhang
May 16, 2008·Human Gene Therapy·Minna U KaikkonenKari J Airenne
Jun 16, 2006·Journal of Virology·Anna R MäkeläChristian Oker-Blom
Nov 25, 2005·Journal of Virology·Heli MatilainenChristian Oker-Blom
Apr 29, 2015·Viruses·Kaisa-Emilia MakkonenSeppo Ylä-Herttulala
Dec 1, 2007·Expert Opinion on Drug Discovery·Robert S AmesJ Patrick Condreay
Jul 6, 2012·Journal of Bioscience and Bioengineering·Tatsuya KatoEnoch Y Park
Aug 4, 2011·Journal of Invertebrate Pathology·Minna U KaikkonenKari J Airenne
Aug 4, 2011·Journal of Invertebrate Pathology·António RoldãoPaula M Alves
Jan 22, 2008·Journal of Virological Methods·Ghiabe-Henri GuibingaTheodore Friedmann
Mar 10, 2007·Angewandte Chemie·Martin Fischlechner, Edwin Donath
Oct 7, 2010·Biotechnology and Applied Biochemistry·Takashi DojimaEnoch Y Park
Oct 7, 2004·Journal of Biotechnology·Kirsi OjalaChristian Oker-Blom
Mar 30, 2006·Journal of Biotechnology·Heli MatilainenChristian Oker-Blom
Mar 18, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ghiabe H Guibinga, Theodore Friedmann
Mar 18, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Linda J NicholsonColin T Dolphin
Jul 21, 2005·Technology in Cancer Research & Treatment·Reetta RiikonenChristian Oker-Blom
Jun 3, 2018·Viruses·Balraj PremanandMookkan Prabakaran
Jun 21, 2018·Molecular Genetics and Genomics : MGG·Hao ZhengJingchen Sun
Feb 12, 2010·Cold Spring Harbor Protocols·Kari J AirenneSeppo Ylä-Herttuala
Mar 3, 2010·Cold Spring Harbor Protocols·Anna R MäkeläChristian Oker-Blom

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