Baculovirus expression of mouse lactoferrin receptor and tissue distribution in the mouse

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Y A Suzuki, B Lönnerdal

Abstract

Lactoferrin (Lf) has been shown to have a role in the immune system and in early development of the mouse embryo. A specific receptor for Lf has been suggested to mediate the functions of Lf. We have recently identified a Lf receptor (LfR) in human fetal small intestine. We therefore hypothesized that the mouse homologue of this protein functions as a LfR. We expressed mouse Lf (MLf) and the mouse homologue (MLfR) in a baculovirus-insect cell system. The recombinant MLfR (rMLfR) was purified by immobilized recombinant MLf (rMLf) affinity chromatography, demonstrating an interaction between rMLf and the rMLfR. RT-PCR revealed that MLfR was expressed in various tissues and during embryonic development. Immunohistochemical analysis revealed that the MLfR was localized in various tissues including small intestinal epithelium, stomach, kidney, ovary, and various regions of brain. In summary, the MLfR functions as a receptor for MLf, is expressed and localized in various tissues, and may be involved in the indispensable function of MLf during early embryonic development.

Citations

Oct 19, 2006·Journal of Biomedical Science·Rong-qin HuangChen Jiang
May 15, 2013·Nature Reviews. Nephrology·A M F MartinesD W Swinkels
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Veronica LopezBo Lönnerdal
Feb 16, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yalin LiaoBo Lönnerdal
Feb 26, 2013·International Journal of Molecular Sciences·Feng-Yun J HuangJem-Mau Lo
Feb 5, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jiarong WangJianhua Wang
Jul 26, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Huali ChenQin He
Dec 23, 2009·Brain Research Bulletin·Rongqin HuangYuanying Pei
Aug 6, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Rongqin HuangYuanying Pei
Sep 4, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yalin LiaoBo Lönnerdal
Aug 4, 2007·International Immunopharmacology·Romy FischerDaniel Tome
Apr 18, 2016·Seminars in Nephrology·Vyvyca J Walker, Anupam Agarwal
Oct 20, 2011·The International Journal of Biochemistry & Cell Biology·Rulan Jiang, Bo Lönnerdal
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Yatao YinYing Peng
Jun 25, 2009·Veterinary Research·Anne T FrenchAlan D Pemberton
Feb 22, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rulan Jiang, Bo Lönnerdal
Apr 19, 2020·Current Pharmaceutical Design·Saurabh MittalSanjula Baboota
Dec 28, 2015·Nanomaterials·Shanshan WangRongqin Huang
Mar 10, 2021·Critical Reviews in Food Science and Nutrition·Michał SienkiewiczJakub Fichna
Jun 15, 2021·The Journal of Veterinary Medical Science·Takahiro AyaTakashi Takeuchi

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